Academic Journal
S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells
| العنوان: | S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells |
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| المؤلفون: | Warner, Matthew J, Bridge, Katherine S, Hewitson, James P, Hodgkinson, Michael R, Heyam, Alex, Massa, Bailey C, Haslam, Jessica C, Chatzifrangkeskou, Maria, Evans, Gareth J O, Plevin, Michael J, Sharp, Tyson V, Lagos, Dimitris |
| سنة النشر: | 2016 |
| المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| الوصف: | MicroRNAs (miRNAs) are short non-coding RNAs that silence mRNAs. They are generated following transcription and cleavage by the DROSHA/DGCR8 and DICER/TRBP/PACT complexes. Although it is known that components of the miRNA biogenesis machinery can be phosphorylated, it remains poorly understood how these events become engaged during physiological cellular activation. We demonstrate that S6 kinases can phosphorylate the extended C-terminal domain of TRBP and interact with TRBP in situ in primary cells. TRBP serines 283/286 are essential for S6K-mediated TRBP phosphorylation, optimal expression of TRBP, and the S6K-TRBP interaction in human primary cells. We demonstrate the functional relevance of this interaction in primary human dermal lymphatic endothelial cells (HDLECs). Angiopoietin-1 (ANG1) can augment miRNA biogenesis in HDLECs through enhancing TRBP phosphorylation and expression in an S6K2-dependent manner. We propose that the S6K2/TRBP node controls miRNA biogenesis in HDLECs and provides a molecular link between the mTOR pathway and the miRNA biogenesis machinery. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://eprints.whiterose.ac.uk/104593/1/NuclAcidsRes_2016_Warner_nar_gkw631.pdf; Warner, Matthew J, Bridge, Katherine S orcid.org/0000-0003-1516-1459 , Hewitson, James P orcid.org/0000-0002-3265-6763 et al. (9 more authors) (2016) S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Research. pp. 9942-9955. ISSN 0305-1048 |
| DOI: | 10.1093/nar/gkw631 |
| الاتاحة: | https://eprints.whiterose.ac.uk/104593/ https://eprints.whiterose.ac.uk/104593/1/NuclAcidsRes_2016_Warner_nar_gkw631.pdf https://doi.org/10.1093/nar/gkw631 |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.E5002DA3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkw631 |
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