التفاصيل البيبلوغرافية
| العنوان: |
Multi-DNA-Modified Double-Network Hydrogel with Customized Microstructure: A Novel System for Living Circulating Tumor Cells Capture and Real-Time Detection |
| المؤلفون: |
Dongliang Chen, Yonggang Li, Xiaoqiu Liu, Yali Zhao, Tianying Ren, Jing Guo, Dawei Yang, Shenghai Li |
| سنة النشر: |
2024 |
| المجموعة: |
Smithsonian Institution: Figshare |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Biotechnology, Immunology, Developmental Biology, Cancer, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, potent fluorescence signature, low degradation rate, growth state even, diagnosing cancer patients, constructed using polyacrylamide, 93 ± 3, 90 ± 2, 67 ± 11, optimal capture efficiencies, cmd grafted onto, 5 cells ml, target cell ’, dense network structure, time fluorescent detection, cmd ), coined, chitosan dn hydrogel, time detection, proficient capture |
| الوصف: |
The precise and effective isolation of living circulating tumor cells (CTCs) from peripheral blood, followed by their real-time monitoring, is crucial for diagnosing cancer patients. In this study, a cell-imprinted double-network (DN) hydrogel modified with circular multi-DNA (CMD), coined the CMD-imprinted hydrogel with fixed cells as templates (CMD-CIDH), was developed. The hydrogel featured a customized surface for proficient capture of viable CTCs and in situ real-time fluorescent detection without subsequent release. The customized surface, constructed using polyacrylamide/chitosan DN hydrogel as the matrix on the cell template, had a dense network structure, thereby ensuring excellent stability and a low degradation rate. Optimal capture efficiencies, recorded at 93 ± 3% for MCF-7 cells and 90 ± 2% for Hela cells, were achieved by grafting the CMD and adjusting the nodule size on the customized surface. The capture efficiency remained significantly high at 67 ± 11% in simulated breast cancer patient experiments even at a minimal concentration of 5 cells mL –1 . Furthermore, CMD grafted onto the surface produced a potent fluorescence signature, enabling in situ real-time fluorescent detection of the target cell’s growth state even in complex environments. The customized surface is highly efficient for screening CTCs in peripheral blood and has promising potential for setting up the CTCs culture. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/media/Multi-DNA-Modified_Double-Network_Hydrogel_with_Customized_Microstructure_A_Novel_System_for_Living_Circulating_Tumor_Cells_Capture_and_Real-Time_Detection/25152810 |
| DOI: |
10.1021/acsami.3c15432.s002 |
| الاتاحة: |
https://doi.org/10.1021/acsami.3c15432.s002 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.E4F04AD8 |
| قاعدة البيانات: |
BASE |