Academic Journal
Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation
| العنوان: | Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation |
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| المؤلفون: | Craveiro, R., Castro, V. I. B., Viciosa, M. T., Dionisio, M., Reis, R. L., Duarte, A. R. C., Paiva, A. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2021 |
| المجموعة: | Universidade of Minho: RepositóriUM |
| مصطلحات موضوعية: | Cryopreservation, Natural Deep-eutectic solvents, Water mixtures, NADES, Glucose, Urea, Proline, Cytotoxicity, Thermal properties, Engenharia e Tecnologia::Biotecnologia Ambiental, Science & Technology |
| الوصف: | Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sci-ences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose,urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and addedin different mass fractions to water.By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even forlow fraction of NADES added. This is also observed by polarized optical microscopy which allowed followingthe formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown asthe crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight frac-tion of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7.NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared withthe commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability testswith cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability.This work combines thermophysical data on NADES and evaluates itâ s in vitro performance, providing cues fortheir use in cryopreservation applications. ; European Union Horizon 2020 Program under the agreement number ERC-2016-CoG 725034 (ERC Consolidator Grant Des.solve). This research was also funded by PTDC/EQU-EQU/29851/2017. A. Paiva acknowledges the financial support from project IF/01146/2015 attributed within the 2015 FCT researcher program. This work was supported by the Associate Laboratory for Green Chemistry - LAQV which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0167-7322 |
| Relation: | info:eu-repo/grantAgreement/EC/H2020/725034/EU; PTDC/EQU-EQU/29851/2017; UIDB/50006/2020; UIDP/50006/2020; https://www.sciencedirect.com/science/article/pii/S0167732221002592; Craveiro R., Castro V. I. B., Viciosa M. T., Dionisio M., Reis R. L., Duarte A. R. C., Paiva A. Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation, Journal of Molecular Liquids, Vol. 329, pp. 115533, doi:10.1016/j.molliq.2021.115533, 2021; http://hdl.handle.net/1822/73487 |
| DOI: | 10.1016/j.molliq.2021.115533 |
| الاتاحة: | http://hdl.handle.net/1822/73487 https://doi.org/10.1016/j.molliq.2021.115533 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.E4BFC304 |
| قاعدة البيانات: | BASE |
| تدمد: | 01677322 |
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| DOI: | 10.1016/j.molliq.2021.115533 |