Academic Journal
Replacing Acid-Heat Precipitation with Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis Improves Detecting Bence-Jones proteinuria
| العنوان: | Replacing Acid-Heat Precipitation with Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis Improves Detecting Bence-Jones proteinuria |
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| المؤلفون: | amirzargar, aliakbar |
| المصدر: | Archives of Medical Laboratory Sciences; Vol. 1 No. 3 (2015): Fall ; 2476-7670 ; 2423-6241 |
| بيانات النشر: | Iranian Scientific Association of Clinical Laboratory |
| سنة النشر: | 2019 |
| المجموعة: | Portal of Scientific Journals at Shahid Beheshti University of Medical Science |
| مصطلحات موضوعية: | Bence-Jones protein, Monoclonal gammopathies, Capillary immuno-electrophoresis, Sodium dodecyl sulphate-polyacrylamide gel electrophoresis |
| الوصف: | Background: Detecting Bence-Jones protein in urine is essential for determining plasma cell dyscrasia and multiple myeloma. Conventionally, acid-heat precipitation assay is used for detecting Bence-jones protein in most medical laboratories; however, because of the low accuracy of this test, other more sensitive tests like urine electrophoresis are recommended.Materials and Methods: In this study, the presence of Bence-jones protein in the urine of patients suspected to monoclonal gammopathies were compared using acid-heat precipitation, capillary immunoelectrophoresis and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Moreover, the subsets of light chain (κ & λ) in capillary immunoelectrophoresis were determined.Results: Our data showed high false negative results (77.7%) using acid-heat precipitation assay in comparison with polyacrylamide gel and capillary immunoelectrophoresis (0%).Conclusion: Collectively, in spite of advantages like easy performance and low cost, acid-heat precipitation assay is not reliable for determining Bence-jones proteinuria in medical laboratories due to its low sensitivity. Therefore, it is recommended to be replaced with more sensitive assays like electrophoresis. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://journals.sbmu.ac.ir/archives/article/view/10875/18161; https://journals.sbmu.ac.ir/archives/article/view/10875 |
| DOI: | 10.22037/amls.v1i3.10875 |
| الاتاحة: | https://journals.sbmu.ac.ir/archives/article/view/10875 https://doi.org/10.22037/amls.v1i3.10875 |
| Rights: | Copyright (c) 2018 Archives of Medical Laboratory Sciences |
| رقم الانضمام: | edsbas.E4BE7529 |
| قاعدة البيانات: | BASE |
| DOI: | 10.22037/amls.v1i3.10875 |
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