Academic Journal

The Virion-Associated Open Reading Frame 49 of Murine Gammaherpesvirus 68 Promotes Viral Replication both In Vitro and In Vivo as a Derepressor of RTA

التفاصيل البيبلوغرافية
العنوان: The Virion-Associated Open Reading Frame 49 of Murine Gammaherpesvirus 68 Promotes Viral Replication both In Vitro and In Vivo as a Derepressor of RTA
المؤلفون: Noh, Cheol-Woo, Cho, Hye-Jeong, Kang, Hye-Ri, Jin, Hyun Yong, Lee, Shaoying, Deng, Hongyu, Wu, Ting-Ting, Arumugaswami, Vaithilingaraja, Sun, Ren, Song, Moon Jung
المصدر: Journal of Virology ; volume 86, issue 2, page 1109-1118 ; ISSN 0022-538X 1098-5514
بيانات النشر: American Society for Microbiology
سنة النشر: 2012
الوصف: Replication and transcription activator (RTA), an immediate-early gene, is a key molecular switch to evoke lytic replication of gammaherpesviruses. Open reading frame 49 (ORF49) is conserved among gammaherpesviruses and shown to cooperate with RTA in regulating virus lytic replication. Here we show a molecular mechanism and in vivo functions of murine gammaherpesvirus 68 (MHV-68 or γHV-68) ORF49. MHV-68 ORF49 was transcribed and translated as a late gene. The ORF49 protein was associated with a virion, interacting with the ORF64 large tegument protein and the ORF25 capsid protein. Moreover, ORF49 directly bound to RTA and its negative cellular regulator, poly(ADP-ribose) polymerase-1 (PARP-1), and disrupted the interactions of RTA and PARP-1. Productive replication of an ORF49-deficient mutant virus (49S) was attenuated in vivo as well as in vitro . Likewise, latent infection was also impaired in the spleen of 49S-infected mice. Taken together, our results suggest that the virion-associated ORF49 protein may promote virus replication both in vitro and in vivo by providing an optimal environment in the early phase of virus infection as a derepressor of RTA.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1128/jvi.05785-11
DOI: 10.1128/JVI.05785-11
الاتاحة: http://dx.doi.org/10.1128/jvi.05785-11
https://journals.asm.org/doi/pdf/10.1128/JVI.05785-11
Rights: https://journals.asm.org/non-commercial-tdm-license
رقم الانضمام: edsbas.E4B67D4
قاعدة البيانات: BASE