Academic Journal
TOPAS-Tissue: A Framework for the Simulation of the Biological Response to Ionizing Radiation at the Multi-Cellular Level.
| العنوان: | TOPAS-Tissue: A Framework for the Simulation of the Biological Response to Ionizing Radiation at the Multi-Cellular Level. |
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| المؤلفون: | García García, Omar, Ortiz, Ramon, Moreno-Barbosa, Eduardo, D-Kondo, Naoki, Faddegon, Bruce, Ramos-Méndez, Jose |
| المصدر: | International Journal of Molecular Sciences, vol 25, iss 18 |
| بيانات النشر: | eScholarship, University of California |
| سنة النشر: | 2024 |
| المجموعة: | University of California: eScholarship |
| مصطلحات موضوعية: | Monte Carlo track-structure simulation, PC-3 cell survival, agent-based modeling, multiscale modeling, radiation damage response, Humans, Monte Carlo Method, Radiation, Ionizing, DNA Repair, Computer Simulation, Models, Biological, Cell Survival, DNA Damage, Dose-Response Relationship, Cell Line, Tumor, DNA Breaks, Double-Stranded |
| الوصف: | This work aims to develop and validate a framework for the multiscale simulation of the biological response to ionizing radiation in a population of cells forming a tissue. We present TOPAS-Tissue, a framework to allow coupling two Monte Carlo (MC) codes: TOPAS with the TOPAS-nBio extension, capable of handling the track-structure simulation and subsequent chemistry, and CompuCell3D, an agent-based model simulator for biological and environmental behavior of a population of cells. We verified the implementation by simulating the experimental conditions for a clonogenic survival assay of a 2-D PC-3 cell culture model (10 cells in 10,000 µm2) irradiated by MV X-rays at several absorbed dose values from 0-8 Gy. The simulation considered cell growth and division, irradiation, DSB induction, DNA repair, and cellular response. The survival was obtained by counting the number of colonies, defined as a surviving primary (or seeded) cell with progeny, at 2.7 simulated days after irradiation. DNA repair was simulated with an MC implementation of the two-lesion kinetic model and the cell response with a p53 protein-pulse model. The simulated survival curve followed the theoretical linear-quadratic response with dose. The fitted coefficients α = 0.280 ± 0.025/Gy and β = 0.042 ± 0.006/Gy2 agreed with published experimental data within two standard deviations. TOPAS-Tissue extends previous works by simulating in an end-to-end way the effects of radiation in a cell population, from irradiation and DNA damage leading to the cell fate. In conclusion, TOPAS-Tissue offers an extensible all-in-one simulation framework that successfully couples Compucell3D and TOPAS for multiscale simulation of the biological response to radiation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | qt7b06t625; https://escholarship.org/uc/item/7b06t625; https://escholarship.org/content/qt7b06t625/qt7b06t625.pdf |
| DOI: | 10.3390/ijms251810061 |
| الاتاحة: | https://escholarship.org/uc/item/7b06t625 https://escholarship.org/content/qt7b06t625/qt7b06t625.pdf https://doi.org/10.3390/ijms251810061 |
| Rights: | public |
| رقم الانضمام: | edsbas.E4B4157B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms251810061 |
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