Academic Journal
Sphingolipid-Induced Programmed Cell Death is a Salicylic Acid and EDS1-Dependent Phenotype in Arabidopsis fatty acid hydroxylase (fah1, fah2) and ceramide synthase (loh2) Triple Mutants
| العنوان: | Sphingolipid-Induced Programmed Cell Death is a Salicylic Acid and EDS1-Dependent Phenotype in Arabidopsis fatty acid hydroxylase (fah1, fah2) and ceramide synthase (loh2) Triple Mutants |
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| المؤلفون: | König, Stefanie, Gömann, Jasmin, Zienkiewicz, Agnieszka, Zienkiewicz, Krzysztof, Meldau, Dorothea, Herrfurth, Cornelia, Feussner, Ivo |
| سنة النشر: | 2021 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | Ceramides and long chain bases (LCBs) are plant sphingolipids involved in the induction of plant programmed cell death (PCD). The fatty acid hydroxylase mutant fah1 fah2 exhibits high ceramide levels and moderately elevated LCB levels. Salicylic acid glucoside (SAG) level is increased in this mutant, but no cell death can be detected by trypan blue staining. To determine the effect of ceramides with different chain lengths, fah1 fah2 was crossed with ceramide synthase mutants longevity assurance gene one homologue1-3 (loh1, loh2 and loh3). Surprisingly, only triple mutants with loh2 show cell death detected by trypan blue staining under the selected conditions. Sphingolipid profiling revealed that the greatest differences between the triple mutant plants are in the LCB and LCB-phosphate (LCB-P) fraction. fah1 fah2 loh2 plants accumulate LCB d18:0, LCB t18:0and LCB-P d18:0. Crossing fah1 fah2 loh2 with the SA synthesis mutant sid2-2, and with the SA signaling mutants enhanced disease susceptibility 1-2 (eds1-2) and phytoalexin deficient 4-1 (pad4-1), revealed that lesions are SA- and EDS1-dependent. These quadruple mutants also confirm that there may be a feedback loop between SA and sphingolipid metabolism as they accumulated less ceramides and LCBs. In conclusion, PCD in fah1 fah2 loh2 is a SA and EDS1-dependent phenotype, which is likely due to accumulation of LCBs. ; Abstract Ceramides and long chain bases (LCBs) are plant sphingolipids involved in the induction of plant programmed cell death (PCD). The fatty acid hydroxylase mutant fah1 fah2 exhibits high ceramide levels and moderately elevated LCB levels. Salicylic acid glucoside (SAG) level is increased in this mutant, but no cell death can be detected by trypan blue staining. To determine the effect of ceramides with different chain lengths, fah1 fah2 was crossed with ceramide synthase mutants longevity assurance gene one homologue1-3 (loh1, loh2 and loh3). Surprisingly, only triple mutants with loh2 show cell death detected by trypan blue ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0032-0781 1471-9053 |
| Relation: | https://resolver.sub.uni-goettingen.de/purl?gro-2/97921 |
| DOI: | 10.1093/pcp/pcab174 |
| الاتاحة: | https://resolver.sub.uni-goettingen.de/purl?gro-2/97921 https://doi.org/10.1093/pcp/pcab174 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.E4ACC603 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00320781 14719053 |
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| DOI: | 10.1093/pcp/pcab174 |