Academic Journal
Saccharomyces cerevisiae is dependent on vesicular traffic between the Golgi apparatus and the vacuole when inositolphosphorylceramide synthase aur1 is inactivated
| العنوان: | Saccharomyces cerevisiae is dependent on vesicular traffic between the Golgi apparatus and the vacuole when inositolphosphorylceramide synthase aur1 is inactivated |
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| المؤلفون: | Voynova, Natalia S, Roubaty, Carole, Vazquez, Hector M, Mallela, Shamroop K, Ejsing, Christer S, Conzelmann, Andreas |
| المصدر: | Voynova , N S , Roubaty , C , Vazquez , H M , Mallela , S K , Ejsing , C S & Conzelmann , A 2015 , ' Saccharomyces cerevisiae is dependent on vesicular traffic between the Golgi apparatus and the vacuole when inositolphosphorylceramide synthase aur1 is inactivated ' , Eukaryotic Cell , vol. 14 , no. 12 , pp. 1203-1216 . https://doi.org/10.1128/EC.00117-15 |
| سنة النشر: | 2015 |
| المجموعة: | University of Southern Denmark: Research Output / Syddansk Universitet |
| مصطلحات موضوعية: | Alleles, Biological Transport/drug effects, Biosynthetic Pathways/drug effects, Ceramides/metabolism, Depsipeptides/pharmacology, Doxycycline/pharmacology, Epistasis, Genetic/drug effects, Gene Deletion, Gene Ontology, Genetic Testing, Glycosphingolipids/metabolism, Golgi Apparatus/drug effects, Hexosyltransferases/antagonists & inhibitors, High-Throughput Screening Assays, Hydrolysis, Lipid Droplets/drug effects, Mutation/genetics, Quinacrine/metabolism, Saccharomyces cerevisiae Proteins/metabolism, Saccharomyces cerevisiae/cytology, Sphingolipids/biosynthesis, Transport Vesicles/drug effects, Vacuoles/drug effects |
| الوصف: | Inositolphosphorylceramide (IPC) and its mannosylated derivatives are the only complex sphingolipids of yeast. Their synthesis can be reduced by aureobasidin A (AbA), which specifically inhibits the IPC synthase Aur1. AbA reportedly, by diminishing IPC levels, causes endoplasmic reticulum (ER) stress, an increase in cytosolic calcium, reactive oxygen production, and mitochondrial damage leading to apoptosis. We found that when Aur1 is gradually depleted by transcriptional downregulation, the accumulation of ceramides becomes a major hindrance to cell survival. Overexpression of the alkaline ceramidase YPC1 rescues cells under this condition. We established hydroxylated C 26 fatty acids as a reliable hallmark of ceramide hydrolysis. Such hydrolysis occurs only when YPC1 is overexpressed. In contrast, overexpression of YPC1 has no beneficial effect when Aur1 is acutely repressed by AbA. A high-throughput genetic screen revealed that vesicle-mediated transport between Golgi apparatus, endosomes, and vacuole becomes crucial for survival when Aur1 is repressed, irrespective of the mode of repression. In addition, vacuolar acidification becomes essential when cells are acutely stressed by AbA, and quinacrine uptake into vacuoles shows that AbA activates vacuolar acidification. The antioxidant N-acetylcysteine does not improve cell growth on AbA, indicating that reactive oxygen radicals induced by AbA play a minor role in its toxicity. AbA strongly induces the cell wall integrity pathway, but osmotic support does not improve the viability of wild-type cells on AbA. Altogether, the data support and refine current models of AbA-mediated cell death and add vacuolar protein transport and acidification as novel critical elements of stress resistance. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://portal.findresearcher.sdu.dk/da/publications/3200af86-4d39-45fb-8608-8372705c1bde |
| DOI: | 10.1128/EC.00117-15 |
| الاتاحة: | https://portal.findresearcher.sdu.dk/da/publications/3200af86-4d39-45fb-8608-8372705c1bde https://doi.org/10.1128/EC.00117-15 https://findresearcher.sdu.dk/ws/files/129111799/Saccharomyces_cerevisiae_depend_on_vesicular_traffic_between_Golgi_and_vacuole_when_Inositolphosphorylceramide_synthase_Aur1_is_inactivated.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664882/ |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.E47F0E4B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/EC.00117-15 |
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