Academic Journal
Design and application of a lactulose biosensor
| العنوان: | Design and application of a lactulose biosensor |
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| المؤلفون: | Wu, Jieyuan, Jiang, Peixia, Chen, Wei, Xiong, Dandan, Huang, Linglan, Jia, Junying, Chen, Yuanyuan, Jin, Jian-Ming, Tang, Shuang-Yan |
| المصدر: | Scientific Reports ; volume 7, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2017 |
| الوصف: | In this study the repressor of Escherichia coli lac operon, LacI, has been engineered for altered effector specificity. A LacI saturation mutagenesis library was subjected to Fluorescence Activated Cell Sorting (FACS) dual screening. Mutant LacI-L5 was selected and it is specifically induced by lactulose but not by other disaccharides tested (lactose, epilactose, maltose, sucrose, cellobiose and melibiose). LacI-L5 has been successfully used to construct a whole-cell lactulose biosensor which was then applied in directed evolution of cellobiose 2-epimerase (C2E) for elevated lactulose production. The mutant C2E enzyme with ~32-fold enhanced expression level was selected, demonstrating the high efficiency of the lactulose biosensor. LacI-L5 can also be used as a novel regulatory tool. This work explores the potential of engineering LacI for customized molecular biosensors which can be applied in practice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/srep45994 |
| الاتاحة: | http://dx.doi.org/10.1038/srep45994 https://www.nature.com/articles/srep45994.pdf https://www.nature.com/articles/srep45994 http://www.nature.com/doifinder/10.1038/srep45994 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.E45DB456 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/srep45994 |
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