Academic Journal
CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates
| العنوان: | CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates |
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| المساهمون: | Ryanggeun Lee, Moo-Koo Kang, Yong-Jin Kim, Bobae Yang, Hwanyong Shim, Sugyung Kim, Kyungwoo Kim, Chul Min Yang, Byeong-Gyu Min, Woong-Jae Jung, Eun-Chong Lee, Jung-Sik Joo, Gunhee Park, Won-Ki Cho, Hyoung-Pyo Kim, Kim, Hyoung Pyo |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | CCCTC-Binding Factor / metabolism, Cell Cycle Proteins / genetics, Cell Cycle Proteins / metabolism, Chromatin / chemistry, Chromatin / genetics, Chromatin / metabolism, Chromatin Assembly and Disassembly, Epigenesis, Genetic, HCT116 Cells, Humans, Mediator Complex Subunit 1 / genetics, Mediator Complex Subunit 1 / metabolism, RNA Polymerase II / metabolism, Transcription Factors / genetics, Transcription Factors / metabolism |
| الوصف: | CTCF is crucial to the organization of mammalian genomes into loop structures. According to recent studies, the transcription apparatus is compartmentalized and concentrated at super-enhancers to form phase-separated condensates and drive the expression of cell-identity genes. However, it remains unclear whether and how transcriptional condensates are coupled to higher-order chromatin organization. Here, we show that CTCF is essential for RNA polymerase II (Pol II)-mediated chromatin interactions, which occur as hyperconnected spatial clusters at super-enhancers. We also demonstrate that CTCF clustering, unlike Pol II clustering, is independent of liquid-liquid phase-separation and resistant to perturbation of transcription. Interestingly, clusters of Pol II, BRD4, and MED1 were found to dissolve upon CTCF depletion, but were reinstated upon restoration of CTCF, suggesting a potent instructive function for CTCF in the formation of transcriptional condensates. Overall, we provide evidence suggesting that CTCF-mediated chromatin looping acts as an architectural prerequisite for the assembly of phase-separated transcriptional condensates. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0305-1048 1362-4962 |
| Relation: | NUCLEIC ACIDS RESEARCH; J02387; OAK-2022-00083; https://ir.ymlib.yonsei.ac.kr/handle/22282913/187866; T202200153; NUCLEIC ACIDS RESEARCH, Vol.50(1) : 207-226, 2022-01 |
| DOI: | 10.1093/nar/gkab1242 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/187866 https://doi.org/10.1093/nar/gkab1242 |
| Rights: | CC BY-NC-ND 2.0 KR |
| رقم الانضمام: | edsbas.E4180D5B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 03051048 13624962 |
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| DOI: | 10.1093/nar/gkab1242 |