Academic Journal
Expression, purification, crystallization and preliminary X-ray studies of the outer membrane efflux proteins OprM and OprN from Pseudomonas aeruginosa
| العنوان: | Expression, purification, crystallization and preliminary X-ray studies of the outer membrane efflux proteins OprM and OprN from Pseudomonas aeruginosa |
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| المؤلفون: | Broutin, Isabelle, Benabdelhak, Houssain, Moreel, Xavier, Lascombe, Marie-Bernard, Lerouge, Dimitri, Ducruix, Arnaud |
| المساهمون: | Laboratoire de cristallographie et RMN biologiques (LCRB - UMR 8015), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Axe cancer, Université Laval Québec (ULaval)-CHUQ Research Center |
| المصدر: | ISSN: 1744-3091. |
| بيانات النشر: | CCSD International Union of Crystallography |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; OprM and OprN belong to the outer membrane factor family proteins. These $52 kDa proteins are part of the tripartite efflux pumps found in Pseudomonas aeruginosa and are responsible in part for the antibiotic resistance observed in these bacteria. Both proteins have been expressed in Escherichia coli as His-tag proteins and purified accordingly by affinity chromatography in the presence of n-octyl-d-glucopyranoside detergent. OprM and OprN were crystallized using PEG 20 000/ammonium citrate and ammonium sulfate as precipitating agents, respectively. Crystals belong to space group C2, with unit-cell parameters a = 152.6, b = 87.9, c = 355.9 Å , = 98.9 and a = 151.3, b = 87.6, c = 356.5 Å , = 98.1 for OprM and OprN, respectively. Using the ESRF synchrotron-radiation source, OprM diffraction data extended to 3.4 Å . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1107/S1744309105005014 |
| الاتاحة: | https://hal.science/hal-02150027 https://hal.science/hal-02150027v1/document https://hal.science/hal-02150027v1/file/Broutin-actaF-oprM-2005.pdf https://doi.org/10.1107/S1744309105005014 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E37C7D22 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1107/S1744309105005014 |
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