Academic Journal
Stenotrophomonas maltophilia strains from cystic fibrosis patients: genomic variability and molecular characterization of some virulence determinants.
| العنوان: | Stenotrophomonas maltophilia strains from cystic fibrosis patients: genomic variability and molecular characterization of some virulence determinants. |
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| المؤلفون: | Nicoletti, Mauro, Iacobino, Angelo, Prosseda, Gianni, Fiscarelli, Ersilia, Zarrilli, Raffaele, de Carolis, Elena, Petrucca, Andrea, Nencioni, Lucia, Colonna, Bianca, Casalino, Mariassunta |
| المساهمون: | Dipartimento Scienze Biomediche, Università degli studi "G. d'Annunzio" Chieti-Pescara Chieti-Pescara (Ud'A), Dipartimento di Biologia, Università degli Studi Roma Tre = Roma Tre University (ROMA TRE), Department of Biology and Biotechnology "Charles Darwin", Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Università degli Studi di Roma "La Sapienza" = Sapienza University Rome (UNIROMA), U.O. Microbiologia, IRCCS Ospedale Pediatrico Bambino Gesù = Bambino Gesù Children’s Hospital, Dipartimento di Scienze Mediche Preventive, Sezione di Igiene, University of Naples Federico II = Università degli studi di Napoli Federico II, Istituto di Microbiologia, Università cattolica del Sacro Cuore Milano (Unicatt), Department of Public Health and Infectious Diseases |
| المصدر: | ISSN: 1438-4221 ; International Journal of Medical Microbiology ; https://riip.hal.science/pasteur-00975900 ; International Journal of Medical Microbiology, 2011, 301 (1), pp.34-43. ⟨10.1016/j.ijmm.2010.07.003⟩. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2011 |
| المجموعة: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| مصطلحات موضوعية: | MESH: Cluster Analysis, MESH: Cystic Fibrosis, MESH: Molecular Sequence Data, MESH: Molecular Typing, MESH: Phylogeny, MESH: RNA, Ribosomal, 16S, MESH: Sequence Analysis, DNA, MESH: Stenotrophomonas maltophilia, MESH: Virulence Factors, MESH: DNA Fingerprinting, MESH: DNA, Bacterial, MESH: Electrophoresis, Gel, Pulsed-Field, MESH: Gene Expression, MESH: Gram-Negative Bacterial Infections, MESH: Humans, MESH: Locomotion, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; The genetic relatedness of 52 Stenotrophomonas maltophilia strains, collected from various environmental and clinical sources, including cystic fibrosis (CF) patients, as well as the presence and the expression of some virulence-associated genes were studied. Pulsed-field gel electrophoresis (PFGE) analysis identified 47 profiles and three clusters of isolates with an identical PFGE pattern considered to be indistinguishable strains. Restriction fragment length polymorphism of the gyrB gene grouped the 52 strains into nine different profiles. Most CF clinical isolates (29 out of 41) showed profile 1, while the analysis of the hypervariable regions of the 16S rRNA gene revealed five distinct allelic variations, with the majority of CF isolates (23 out of 41) belonging to sequence group 1. Furthermore, the strains were characterized for motility and expression of virulence-associated genes, including genes encoding type-1 fimbriae, proteases (StmPr1 and StmPr2) and esterase. All S. maltophilia strains exhibited a very broad range of swimming and twitching motility, while none showed swarming motility. A complete smf-1 gene was PCR-amplified only from clinically derived S. maltophilia strains. Finally, the virulence of representative S. maltophilia strains impaired in the expression of proteases and esterase activities was evaluated by infecting larvae of the wax moth Galleria mellonella. The results obtained strongly indicate that the major extracellular protease StmPr1 may be a relevant virulence factor of S. maltophilia. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/20952251; PUBMED: 20952251 |
| DOI: | 10.1016/j.ijmm.2010.07.003 |
| الاتاحة: | https://riip.hal.science/pasteur-00975900 https://riip.hal.science/pasteur-00975900v1/document https://riip.hal.science/pasteur-00975900v1/file/Nicoletti2011.pdf https://doi.org/10.1016/j.ijmm.2010.07.003 |
| رقم الانضمام: | edsbas.E31A8A1F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ijmm.2010.07.003 |
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