Knockdown of KIN13-5 causes mis-placement of the cell division plane.

التفاصيل البيبلوغرافية
العنوان: Knockdown of KIN13-5 causes mis-placement of the cell division plane.
المؤلفون: Huiqing Hu, Yasuhiro Kurasawa, Qing Zhou, Ziyin Li
سنة النشر: 2024
المجموعة: Smithsonian Institution: Figshare
مصطلحات موضوعية: Biophysics, Microbiology, Cell Biology, Genetics, Physiology, Developmental Biology, Cancer, Hematology, Physical Sciences not elsewhere classified, specific signaling pathways, providing mechanistic insights, multiple cytoskeletal structures, early branching eukaryote, div >< p, biochemical assays demonstrate, 13 family protein, longitudinal cell axis, cell division plane, cell anterior toward, 5 causes loss, 5 bundles microtubules, promoting microtubule bundling, cytokinesis regulatory pathway, 13 family kinesin, posterior cell tip, trypanosoma brucei <, microtubule bundling, cell posterior, flagellated cell, brucei <
الوصف: ( A ). Scanning electron microscopic analysis of the cell division plane in control and KIN13-5 RNAi cells. Yellow dash-line arrows indicate the cell division plane. EP, existing posterior; NP, nascent posterior. Scale bars: 5 μm. ( B ). Immunostaining of the cleavage furrow/cell division plane with the cleavage furrow marker protein KLIF. KLIF was endogenously tagged with a C-terminal triple HA epitope and detected by FITC-conjugated anti-HA antibody. Scale bar: 5 μm. ( C ). Quantitation of bi-nucleated cells with a mis-placed cell division plane. Error bars indicate S.D. from three independent experimental replicates. ( D ). Measurement of the inter-posterior distance in control and KIN13-5 RNAi cells using ImageJ. ***, p <0.001.
نوع الوثيقة: still image
اللغة: unknown
Relation: https://figshare.com/articles/figure/Knockdown_of_KIN13-5_causes_mis-placement_of_the_cell_division_plane_/25129954
DOI: 10.1371/journal.ppat.1012000.g003
الاتاحة: https://doi.org/10.1371/journal.ppat.1012000.g003
Rights: CC BY 4.0
رقم الانضمام: edsbas.E3127AE3
قاعدة البيانات: BASE
الوصف
DOI:10.1371/journal.ppat.1012000.g003