التفاصيل البيبلوغرافية
العنوان: |
Knockdown of KIN13-5 causes mis-placement of the cell division plane. |
المؤلفون: |
Huiqing Hu, Yasuhiro Kurasawa, Qing Zhou, Ziyin Li |
سنة النشر: |
2024 |
المجموعة: |
Smithsonian Institution: Figshare |
مصطلحات موضوعية: |
Biophysics, Microbiology, Cell Biology, Genetics, Physiology, Developmental Biology, Cancer, Hematology, Physical Sciences not elsewhere classified, specific signaling pathways, providing mechanistic insights, multiple cytoskeletal structures, early branching eukaryote, div >< p, biochemical assays demonstrate, 13 family protein, longitudinal cell axis, cell division plane, cell anterior toward, 5 causes loss, 5 bundles microtubules, promoting microtubule bundling, cytokinesis regulatory pathway, 13 family kinesin, posterior cell tip, trypanosoma brucei <, microtubule bundling, cell posterior, flagellated cell, brucei < |
الوصف: |
( A ). Scanning electron microscopic analysis of the cell division plane in control and KIN13-5 RNAi cells. Yellow dash-line arrows indicate the cell division plane. EP, existing posterior; NP, nascent posterior. Scale bars: 5 μm. ( B ). Immunostaining of the cleavage furrow/cell division plane with the cleavage furrow marker protein KLIF. KLIF was endogenously tagged with a C-terminal triple HA epitope and detected by FITC-conjugated anti-HA antibody. Scale bar: 5 μm. ( C ). Quantitation of bi-nucleated cells with a mis-placed cell division plane. Error bars indicate S.D. from three independent experimental replicates. ( D ). Measurement of the inter-posterior distance in control and KIN13-5 RNAi cells using ImageJ. ***, p <0.001. |
نوع الوثيقة: |
still image |
اللغة: |
unknown |
Relation: |
https://figshare.com/articles/figure/Knockdown_of_KIN13-5_causes_mis-placement_of_the_cell_division_plane_/25129954 |
DOI: |
10.1371/journal.ppat.1012000.g003 |
الاتاحة: |
https://doi.org/10.1371/journal.ppat.1012000.g003 |
Rights: |
CC BY 4.0 |
رقم الانضمام: |
edsbas.E3127AE3 |
قاعدة البيانات: |
BASE |