التفاصيل البيبلوغرافية
| العنوان: |
Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV |
| المؤلفون: |
Dejan Vidanović, Bojana Tešović, Milanko Šekler, Zoran Debeljak, Nikola Vasković, Kazimir Matović, Andrey Koltsov, Kiril Krstevski, Tamaš Petrović, Ilse De Leeuw, Andy Haegeman |
| المصدر: |
Microorganisms; Volume 9; Issue 6; Pages: 1234 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
lumpy skin disease virus, real-time PCR, DIVA diagnostic protocol, differentiation, field strain, vaccine strain |
| جغرافية الموضوع: |
agris |
| الوصف: |
Lumpy skin disease (LSD) is an important animal disease with significant health and economic impacts. It is considered a notifiable disease by the OIE. Attenuated strains of LSDV have been successfully used as vaccines (LAV) but can also produce mild or systemic reactions. Vaccination campaigns using LAVs are therefore only viable if accompanying DIVA assays are available. Two DIVA qPCR assays able to distinguish Neethling-based LAVs and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic performance comparable to other published DIVA assays. This confirmed their potential as reliable tools to confirm infection in animals during vaccination campaigns based on Neethling vaccine strains. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Public Health Microbiology; https://dx.doi.org/10.3390/microorganisms9061234 |
| DOI: |
10.3390/microorganisms9061234 |
| الاتاحة: |
https://doi.org/10.3390/microorganisms9061234 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.E2E417E2 |
| قاعدة البيانات: |
BASE |