Academic Journal
Design of a lipid nano-delivery system containing recombinant Candida albicans chitinase 3 as a potential vaccine against fungal infections
| العنوان: | Design of a lipid nano-delivery system containing recombinant Candida albicans chitinase 3 as a potential vaccine against fungal infections |
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| المؤلفون: | Augusto Costa-Barbosa, Maria Inês Pacheco, Catarina Carneiro, Cláudia Botelho, Andreia C. Gomes, M. Elisabete C.D. Real Oliveira, Tony Collins, Manuel Vilanova, Célia Pais, Alexandra Correia, Paula Sampaio |
| المصدر: | Biomedicine & Pharmacotherapy, Vol 166, Iss , Pp 115362- (2023) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | DODAB:MO nano-delivery system, DOBAB:MO liposomes, Candida albicans Chitinase 3, Antigen immunomodulation, Antifungal vaccine, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Opportunistic fungi cause lethal systemic infections and impose high medical costs to health systems. The World Health Organization has recognized the importance of fungal infections, including them in its global priority list guiding research, development, and discovery of new therapeutic approaches. Fungal vaccine development has been proposed as one of the treatment and prevention strategies in the last decade. In this study, we present the design of a lipid antigen delivery system based on Dioctadecyldimethylammonium bromide: Monoolein (DODAB: MO) containing recombinant Candida albicans Chitinase 3 (Cht3) for modulation the immune response against fungal infections. Several DODAB:MO liposomes containing Cht3 were prepared and those prepared by the incubation method and containing 5 µg/mL Cht3 were selected due to their favorable size, ζ-potential and stability, suited for antigen delivery applications. The encapsulation of Cht3 in these liposomes resulted in a significant increase in cellular uptake compared to empty liposomes, demonstrating their efficacy in delivering the antigen. Moreover, the liposomes proved to be safe for use in immunization procedures. Subcutaneous administration of Cht3 liposomes elicited a Th1/Th17 immune response profile, associated with the production of high levels of antibodies against Cht3. These antibodies recognized both the native and the recombinant forms of the protein, opsonizing mother-yeast at the cell scars, which has the potential to disrupt cell separation and hinder yeast growth. The findings suggest that the designed lipid antigen delivery system shows promise as a potential candidate for enhancing immune responses against fungal infections, offering a valuable strategy for future fungal vaccine development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0753-3322 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0753332223011538; https://doaj.org/toc/0753-3322; https://doaj.org/article/21630b2d25034943ab90006a6c8a17f6 |
| DOI: | 10.1016/j.biopha.2023.115362 |
| الاتاحة: | https://doi.org/10.1016/j.biopha.2023.115362 https://doaj.org/article/21630b2d25034943ab90006a6c8a17f6 |
| رقم الانضمام: | edsbas.E2816452 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 07533322 |
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| DOI: | 10.1016/j.biopha.2023.115362 |