Dissertation/ Thesis
Novel roles of smallGTPase Rac1 in invasive collective cell migration within tissues ; Nouveaux rôles de small GTPase Rac1 dans la migration cellulaire collective invasive dans les tissus
| العنوان: | Novel roles of smallGTPase Rac1 in invasive collective cell migration within tissues ; Nouveaux rôles de small GTPase Rac1 dans la migration cellulaire collective invasive dans les tissus |
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| المؤلفون: | Zhou, Sijia |
| المساهمون: | Unité de biologie Moléculaire, Cellulaire et du Développement (MCD), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université Paul Sabatier - Toulouse III, Xiaobo Wang |
| المصدر: | https://theses.hal.science/tel-04634664 ; Human health and pathology. Université Paul Sabatier - Toulouse III, 2023. English. ⟨NNT : 2023TOU30022⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2023 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | Collective cell migration, Multicellular environment, Optogenetics, Migration cellulaire collective, Environnement multicellulaire, Optogénétique, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | Collective cell migration plays important roles in development and cancer biology. By using Drosophila border cell migration as an in vivo model, and applying Drosophila genetics, live imaging, optogenetics and physical property measurement, during my PhD study, I aimed at addressing two important questions of collective cell migration within tissues: integration of collective cell direction and coordination; tissue physical property governing collective cell migration. Integration of collective cell direction and coordination ensures collective guidance for efficient movement. I revealed two Rac1 pools at border cell protrusions and supracellular cables, two important structures responsible for direction and coordination. Rac1 and Rho1 form a positive feedback loop that guides mechanical coupling at cables to achieve migration coordination. Rac1 cooperates with Cdc42 to control protrusion growth for migration direction, as well as to regulate F-actin protrusion-cable exchange, linking direction and coordination. Chemokine receptors PVR and EGFR guide correct Rac1 activity distribution at protrusions and cables, thus emphasizing the existence of a balance between two Rac1 pools to achieve collective guidance. Different from matrix environment, environment substrate cells use invasive gaps, enriched with cortical tension continuum, to support border cell migration. I found that Rac1/Rho1 synergistic control on substrate cell cortical tension modifies invasive gaps. And this mechanical property is also interdependent with cytoplasmic pressure in substrate cells. My studies support that substrate cell tension and pressure cooperate in different ways to govern invasive gaps thus controlling border cell migration within tissues. Taken together, I identified two novel functions of small GTPase Rac1 in migrating cells and their surrounding environment. These new findings will facilitate us to better understand invasive collective cell migration during cancer progression. ; La migration cellulaire collective joue un ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| Relation: | NNT: 2023TOU30022; tel-04634664; https://theses.hal.science/tel-04634664; https://theses.hal.science/tel-04634664/document; https://theses.hal.science/tel-04634664/file/2023TOU30022b.pdf |
| الاتاحة: | https://theses.hal.science/tel-04634664 https://theses.hal.science/tel-04634664/document https://theses.hal.science/tel-04634664/file/2023TOU30022b.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E2278F62 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |