Academic Journal
Assessing and Engineering Antibody Stability Using Experimental and Computational Methods
| العنوان: | Assessing and Engineering Antibody Stability Using Experimental and Computational Methods |
|---|---|
| المؤلفون: | Zhang, Cheng, Dalby, Paul Anthony |
| المصدر: | Methods in Molecular Biology , 2552 pp. 165-197. (2023) |
| بيانات النشر: | Humana Press |
| سنة النشر: | 2023 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Antibodies, Antibody Affinity, Immunoglobulin Fab Fragments, Molecular Conformation, Protein Engineering, Protein Stability, Aggregation, Antibody, B-factor, Entropy, Fab, Molecular dynamics (Gromacs), Rosetta, Tm |
| الوصف: | Engineering increased stability into antibodies can improve their developability. While a range of properties need to be optimized, thermal stability and aggregation are two key factors that affect the antibody yield, purity, and specificity throughout the development and manufacturing pipeline. Therefore, an ideal goal would be to apply protein engineering methods early-on, such as in parallel to affinity maturation, to screen out potential drug molecules with the desired conformational and colloidal stability. This chapter introduces our methods to computationally characterize an antibody Fab fragment, propose stabilizing variants, and then experimentally verify these predictions. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://discovery.ucl.ac.uk/id/eprint/10159884/1/2020-05-23%20version.pdf; https://discovery.ucl.ac.uk/id/eprint/10159884/ |
| الاتاحة: | https://discovery.ucl.ac.uk/id/eprint/10159884/1/2020-05-23%20version.pdf https://discovery.ucl.ac.uk/id/eprint/10159884/ |
| Rights: | open |
| رقم الانضمام: | edsbas.E1CEBB5D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |