Academic Journal
PF511 DISSECTING THE MOLECULAR PROFILES IN DIFFUSE LARGE B‐CELL LYMPHOMA USING THE RNA‐ AND PROTEIN‐BARCODED NCOUNTER METHOD
| العنوان: | PF511 DISSECTING THE MOLECULAR PROFILES IN DIFFUSE LARGE B‐CELL LYMPHOMA USING THE RNA‐ AND PROTEIN‐BARCODED NCOUNTER METHOD |
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| المؤلفون: | Chen, C.‐C., Hsu, C.‐C., Huang, C.‐E., Chen, Y.‐J., Chen, Y.‐Y., Wu, Y.‐Y., Tsou, H.‐Y., Tsai, P.‐W., Li, C.‐P., Chuang, W.‐H., Lai, Y.‐H., Lin, Y.‐H. |
| المصدر: | HemaSphere ; volume 3, issue S1, page 208 ; ISSN 2572-9241 2572-9241 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background: Diffuse large B‐cell lymphoma (DLBCL), though highly curable with immunochemotherapy, is a heterogenous group of diseases that include subsets of tumors with different cells of origin (COO). Using gene‐expression profiling, DLBCLs can be categorized into 2 clinically distinct subgroups, namely activated B‐cell (ABC) and germinal center B‐cell (GCB). More recently, double‐expressor (DEL) or double‐hit lymphoma (DHL) was described as a new form of aggressive lymphoma based on the expression or re‐arrangement of 3 genes ( MYC, BCL2, and BCL6 ), respectively. It is speculated that divergent signaling pathways are utilized among DLBCLs with different subtypes, and heterogeneity might even exist in the same subcategory. Aims: To improve our understanding in DLBCL biology, we aim to explore the signaling activities of tumor cells at the mRNA transcripts and protein levels in a cohort of patients with DLBCL. Methods: We enrolled 60 DLBCLs and 6 healthy adults in this study. The DLBCL samples were supplied in FFPE sections while the healthy samples were collected with fresh buffy coats followed by positive selection of pan‐B cells. RNA was extracted using the ReliaPrep™ FFPE kit, and digital GEP was performed to assess the expression of 240 genes using a barcoding profiling method (nCounter technology, NanoString). After data processing with nSolver4.0, the geometric mean expression of all house‐keeping genes was used as an inter‐sample normalization factor and the levels of expression in DLBCL cells were presented in log 2 (fold changes). The barcoded protein profiling was performed with nCounter ® Vantage 3D™ Protein Panels which detected 35 proteins in one FFPE slide. The raw values of protein expression were subtracted with isotype background, normalized with house‐keeping proteins, and similarly presented in log 2 (fold change). Results: The nCounter technology successfully classified samples into ABC and GCB subtypes. Outcome analysis unequivocally demonstrated a worse overall survival in those with ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1097/01.hs9.0000560144.02185.47 |
| DOI: | 10.1097/01.HS9.0000560144.02185.47 |
| الاتاحة: | http://dx.doi.org/10.1097/01.hs9.0000560144.02185.47 https://onlinelibrary.wiley.com/doi/pdf/10.1097/01.HS9.0000560144.02185.47 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.E1C69BC2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/01.hs9.0000560144.02185.47 |
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