التفاصيل البيبلوغرافية
| العنوان: |
Pressure-Jump Kinetics of Liquid–Liquid Phase Separation: Comparison of Two Different Condensed Phases of the RNA-Binding Protein, Fused in Sarcoma |
| المؤلفون: |
Ryo Kitahara (1852906), Ryota Yamazaki (11009111), Fumika Ide (11718457), Shujie Li (2902547), Yutaro Shiramasa (11718460), Naoya Sasahara (11718463), Takuya Yoshizawa (11009108) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Marine Biology, Infectious Diseases, Space Science, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, stronger intermolecular interaction, reaction rate constant, physiological maturation process, might promote transformation, llps vanishing rate, another liquid phase, growth rates decreased, constant growth rate, assembled liquid droplets, vanishing rates indicates, nucleation rate decreased, llps states could, avrami exponent obtained, llps formation rate, llps formation (<, reversible liquid droplets, llps states observed, binding protein fused, binding protein, avrami exponent, reversible hydrogels, llps occurs, could thus |
| الوصف: |
The RNA-binding protein fused in sarcoma (FUS) undergoes liquid–liquid phase separation (LLPS) both in vivo and in vitro . Self-assembled liquid droplets of FUS transform into reversible hydrogels and into more irreversible and toxic aggregates. Although LLPS can be a precursor of irreversible aggregates, a generic method to study kinetics of the formation of LLPS has not been developed. Here, we demonstrated the pressure-jump kinetics of phase transition between the 1-phase state and FUS-LLPS states observed at low pressure (<2 kbar, LP-LLPS) and high pressure (>2 kbar, HP-LLPS) using high-pressure UV/vis spectroscopy. Absorbance (turbidity) changes were reproduced repeatedly using pressure cycles. The Johnson–Mehl–Avrami–Kolmogorov theory was used to understand droplet formation occurring via nucleation and growth. The Avrami exponent n , representing the dimensionality of growing droplets, and the reaction rate constant k were calculated. The HP-LLPS formation rate was ∼2-fold slower than that of LP-LLPS. The Avrami exponent obtained for both LLPS states could be explained by diffusion-limited growth. Nucleation and growth rates decreased during LP-LLPS formation ( n = 0.51), and the nucleation rate decreased with a constant growth rate in HP-LLPS formation ( n = 1.4). The HP-LLPS vanishing rate was ∼20-fold slower than that of LP-LLPS. This difference in vanishing rates indicates a stronger intermolecular interaction in HP-LLPS than in LP-LLPS, which might promote transformation into irreversible aggregates in the droplets. Further, direct transition from HP-LLPS to LP-LLPS was observed. This indicates that interconversion between LP-LLPS and HP-LLPS occurs in equilibrium. Formation of reversible liquid droplets, followed by phase transition into another liquid phase, could thus be part of the physiological maturation process of FUS-LLPS. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Pressure-Jump_Kinetics_of_Liquid_Liquid_Phase_Separation_Comparison_of_Two_Different_Condensed_Phases_of_the_RNA-Binding_Protein_Fused_in_Sarcoma/17032948 |
| DOI: |
10.1021/jacs.1c07571.s001 |
| الاتاحة: |
https://doi.org/10.1021/jacs.1c07571.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.E178782A |
| قاعدة البيانات: |
BASE |