Academic Journal
Isolation and characterization of mesenchymal stem cells derived from horse synovial liquid presenting the STRO-1 surface marker ; Aislamiento y caracterización de las células madre mesenquimales derivadas de líquido sinovial de caballo que presentan el marcador de superficie STRO-1
| العنوان: | Isolation and characterization of mesenchymal stem cells derived from horse synovial liquid presenting the STRO-1 surface marker ; Aislamiento y caracterización de las células madre mesenquimales derivadas de líquido sinovial de caballo que presentan el marcador de superficie STRO-1 |
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| المؤلفون: | Gonzales Figueroa, Hugo, Sen Wong, Yat, Gonzales Molfino, Hugo Mauricio, Llanos Carrillo, José Luis |
| المصدر: | Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 6 (2021); e21682 ; Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 6 (2021); e21682 ; 1682-3419 ; 1609-9117 |
| بيانات النشر: | Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| سنة النشر: | 2021 |
| المجموعة: | Universidad Nacional Mayor de San Marcos: Revistas de investigación UNMSM |
| مصطلحات موضوعية: | mesenchymal stem cells, MSC, horse synovial fluid, biomarker, STRO-1, OCT4, NANOG, células madre mesenquimales, CMM, líquido sinovial de caballos, biomarcador |
| الوصف: | The biological properties of mesenchymal stem cells (MSCs) from synovial fluid from horse limb joints were analysed. The samples were characterized by their peculiarity of adhering to the plastic of the culture flasks. The electromagnetic field technique (MACS) and the surface biomarker STRO-1 were used to optimize their isolation by enriching mesenchymal precursor cells (MSC-LS). To determine the expression of multipotency genes, total RNA was extracted and M-MLV reverse transcriptase was used for the cDNA, which served as a template for qPCR (quantitative PCR). qPCR was performed using the BioRad system with Sybr Green quantification. The multipotency genes quantified were OCT4 and NANOG. The cells selected using MACS with the surface biomarker STRO-1 (CMM-LS STRO-1) proliferate more rapidly than the CMM-LS obtained without purification. In addition, they showed a greater predisposition to differentiate into chondrocytes and osteocytes compared to non-purified LS-MSCs, and a lower predisposition to differentiate into adipocytes. Likewise, the expression of OCT-4 was significantly higher in the CMM-LS STRO-1 compared to the non-purified CMM-LS. NANOG expression was slightly higher in STRO-1 CMM-LS, but without significant differences. The results suggest that the co-expression of OCT-4 and Nanog could increase the physiological functions of MSC-LS STRO-1 related to better proliferation, self-renewal, and the predisposition to differentiate in osteochondral lineages; however, the functional role of these pluripotency markers in adult stem cells needs to be clarified. ; Se analizaron las propiedades biológicas de las células madre mesenquimales (CMM) provenientes del líquido sinovial de las articulaciones de las extremidades de caballo. Las muestras fueron caracterizadas por su peculiaridad de adherirse al plástico de los frascos de cultivos. Se utilizó la técnica de campos electromagnéticos (MACS) y el biomarcador de superficie STRO-1 con la finalidad de optimizar su aislamiento mediante el enriquecimiento de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Spanish; Castilian |
| Relation: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21682/17433; https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21682 |
| DOI: | 10.15381/rivep.v32i6.21682 |
| الاتاحة: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21682 https://doi.org/10.15381/rivep.v32i6.21682 |
| Rights: | Derechos de autor 2021 Hugo Gonzales Figueroa, Yat Sen Wong, Hugo Mauricio Gonzales Molfino, José Luis Llanos Carrillo ; http://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.E13F115C |
| قاعدة البيانات: | BASE |
| DOI: | 10.15381/rivep.v32i6.21682 |
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