Academic Journal
Tailoring a Minimal Self‐Replicate DNA Circuit for Highly Efficient Intracellular Imaging of microRNA
| العنوان: | Tailoring a Minimal Self‐Replicate DNA Circuit for Highly Efficient Intracellular Imaging of microRNA |
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| المؤلفون: | Wei, Jie, Yu, Mengdi, Tan, Kaiyue, Shang, Jinhua, He, Shizhen, Xie, Chenxia, Liu, Xiaoqing, Wang, Fuan |
| المساهمون: | National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities |
| المصدر: | Small ; volume 19, issue 17 ; ISSN 1613-6810 1613-6829 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2023 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Trace analyte detection in complex intracellular environment requires the development of simple yet robust self‐sufficient molecular circuits with high signal‐gain and anti‐interference features. Herein, a minimal non‐enzymatic self‐replicate DNA circuitry (SDC) system is proposed with high‐signal‐gain for highly efficient biosensing in living cells. It is facilely engineered through the self‐stacking of only one elementary cascade hybridization reaction (CHR), thus is encoding with more economic yet effective amplification pathways and reactants. Trigger ( T ) stimulates the activation of CHR for producing numerous T replica that reversely motivate new CHR reaction cycles, thus achieving the successive self‐replication of CHR system with an exponentially magnified readout signal. The intrinsic self‐replicate circuity design and the self‐accelerated reaction format of SDC system is experimentally demonstrated and theoretically simulated. With simple circuitry configuration and low reactant complexity, the SDC amplifier enables the high‐contrast and accurate visualization of microRNA (miRNA), ascribing to its robust molecular recognition and self‐sufficient signal amplification, thus offering a promising strategy for monitoring these clinically significant analytes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/smll.202207961 |
| الاتاحة: | http://dx.doi.org/10.1002/smll.202207961 https://onlinelibrary.wiley.com/doi/pdf/10.1002/smll.202207961 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/smll.202207961 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.E120E97 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/smll.202207961 |
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