Academic Journal
Alginate Encapsulation of Begonia Microshoots for Short‐Term Storage and Distribution
| العنوان: | Alginate Encapsulation of Begonia Microshoots for Short‐Term Storage and Distribution |
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| المؤلفون: | Sakhanokho, Hamidou F., Pounders, Cecil T., Blythe, Eugene K. |
| المساهمون: | Kocsy, G., Sarkar, D., Wilson, C. R. |
| المصدر: | The Scientific World Journal ; volume 2013, issue 1 ; ISSN 2356-6140 1537-744X |
| بيانات النشر: | Wiley |
| سنة النشر: | 2013 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Synthetic seeds were formed from shoot tips of two in vitro grown Begonia cultivars using 3% sodium alginate in Murashige and Skoog medium (MS) salt solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by releasing the sodium alginate/explant combination into 100 mM calcium chloride (CaCl 2 ·H 2 O) solution for 30 or 45 min. Both control and encapsulated shoots were transferred into sterile Petri dishes and stored at 4°C or 22°C for 0, 2, 4, 6, or 8 weeks. Conversion of synthetic seeds into plantlets for both storage environments was assessed in MS medium or peat‐based substrate. No significant difference was found between the 30 and 45 min CaCl 2 ·H 2 O treatments or the two cultivars. Encapsulation of explants improved survival rate over time irrespective of the medium type or storage environment. Survival rates of 88, 53, 28, and 11% for encapsulated microshoots versus 73, 13, 0, and 0% for control explants were achieved in microshoots stored for 2, 4, 6, and 8 weeks, respectively. The best results were obtained when synthetic seeds were stored at 4°C and germinated on MS medium. Regenerated plantlets were successfully established in potting soil. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1155/2013/341568 |
| الاتاحة: | http://dx.doi.org/10.1155/2013/341568 http://downloads.hindawi.com/journals/tswj/2013/341568.pdf http://downloads.hindawi.com/journals/tswj/2013/341568.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2013/341568 |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| رقم الانضمام: | edsbas.E10F32B0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2013/341568 |
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