Academic Journal
Polymerase chain reaction for diagnosing pneumocystis pneumonia in non-HIV immunocompromised patients with pulmonary infiltrates.
| العنوان: | Polymerase chain reaction for diagnosing pneumocystis pneumonia in non-HIV immunocompromised patients with pulmonary infiltrates. |
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| المؤلفون: | Azoulay, Elie, Bergeron, Anne, Chevret, Sylvie, Bele, Nicolas, Schlemmer, Benoît, Menotti, Jean |
| المساهمون: | Service de réanimation médicale, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal Paris, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Pulmonology Department, Biostatistique et épidemiologie clinique, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | ISSN: 0012-3692. |
| بيانات النشر: | HAL CCSD American College of Chest Physicians |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | MESH: Bronchoalveolar Lavage Fluid, MESH: HIV Seronegativity, MESH: Sputum, MESH: Humans, MESH: Immunocompromised Host, MESH: Opportunistic Infections, MESH: Pneumocystis jirovecii, MESH: Pneumonia, Pneumocystis, MESH: Polymerase Chain Reaction, MESH: Predictive Value of Tests, MESH: Sensitivity and Specificity, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
| الوصف: | International audience ; RATIONALE: Pneumocystis jiroveci polymerase chain reaction (PCR) has higher sensitivity than conventional stains but cannot distinguish colonization from infection. METHODS: We compared P jiroveci PCR and conventional stains in HIV-uninfected immunocompromised patients. RESULTS: Among the 448 patients, 296 (66%) patients had hematologic malignancies; 72 (16.1%), bone marrow transplants; 44 (9.8%), solid tumors; 21 (4.7%), renal transplants; and 15 (3.4%) were taking immunosuppressants for systemic diseases. Diagnostic strategy consisted of BAL in 351 patients and induced sputum (IS) in 97 patients. Conventional pneumocystic pneumonia (PCP) stain was positive in 39 (8.7%) patients, including 34 with positive PCR. In addition, PCR was positive in 32 patients, including 21 with complete follow-up, of whom 14 were diagnosed with probable or definitive PCP (a 36% increase). PCR was 87.2% sensitive and 92.2% specific; positive and negative predictive values were 51.5% and 98.7%, respectively. Sensitivity and negative predictive value were 100% on IS. CONCLUSIONS: In HIV-uninfected immunocompromised patients with acute pulmonary infiltrates, P jiroveci PCR correlates with clinical evidence of PCP. A negative PCR allows withdrawing anti-PCP therapy. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19265086; inserm-00448960; https://inserm.hal.science/inserm-00448960; PUBMED: 19265086 |
| DOI: | 10.1378/chest.08-1309 |
| الاتاحة: | https://inserm.hal.science/inserm-00448960 https://doi.org/10.1378/chest.08-1309 |
| رقم الانضمام: | edsbas.E07F5184 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1378/chest.08-1309 |
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