Academic Journal
Molecular basis for the effects of zinc deficiency on spermatogenesis: An experimental study in the Sprague-dawley rat model
| العنوان: | Molecular basis for the effects of zinc deficiency on spermatogenesis: An experimental study in the Sprague-dawley rat model |
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| المؤلفون: | Alexander E Omu, Majedah K Al-Azemi, May Al-Maghrebi, Chacko T Mathew, Florence E Omu, Elijah O Kehinde, Jehoram T Anim, Mabayoje A Oriowo, Anjum Memon |
| المصدر: | Indian Journal of Urology, Vol 31, Iss 1, Pp 57-64 (2015) |
| بيانات النشر: | Wolters Kluwer Medknow Publications |
| سنة النشر: | 2015 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Apoptosis, oxidative stress, sex hormones, spermatogenesis, zinc, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Introduction: The objective of this study is to investigate the molecular mechanisms underlying the effects of zinc deficiency on spermatogenesis in the Sprague-Dawley (SD) rat. Materials and Methods: Three groups of eight adult male SD rats were maintained for 4 weeks on a normal diet as control, zinc deficient diet and zinc deficient diet with zinc supplementation of 28 mg zinc/kg body weight respectively. Using standard techniques, the following parameters were compared between the three groups of experimental animals at the end of 4 weeks: (a) Serum zinc, magnesium (Mg), copper (Cu), selenium (Se) and cadmium (Cd), (b) serum sex hormones, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPX), (c) interleukin-4 (IL-4), tumor necrosis factor-alpha (TNF-α), Bcl-2, Bax and caspase-3 expression in the testes, (d) assessment of apoptosis of testicular cells using electron microscopy and (e) testicular volume and histology using the orchidometer and Johnsen score, respectively. Results: The zinc deficient group showed a reduction of testicular volume, serum concentrations of Zn, Cu, Se, Mg, SOD, GPX, IL-4, Bcl-2 and testosterone (P < 0.05), as well as increased levels of serum Cd, MDA and tissue TNF-α, Bax, caspase-3 and apoptosis of the germ cells (P < 0.05) compared with control and zinc supplementation groups. Conclusion: Zinc deficiency is associated with impaired spermatogenesis because of reduced testosterone production, increased oxidative stress and apoptosis. These findings suggest that zinc has a role in male reproduction. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0970-1591 1998-3824 |
| Relation: | http://www.indianjurol.com/article.asp?issn=0970-1591;year=2015;volume=31;issue=1;spage=57;epage=64;aulast=Omu; https://doaj.org/toc/0970-1591; https://doaj.org/toc/1998-3824; https://doaj.org/article/8a0b282731fe401881a86d393ca64f96 |
| DOI: | 10.4103/0970-1591.139570 |
| الاتاحة: | https://doi.org/10.4103/0970-1591.139570 https://doaj.org/article/8a0b282731fe401881a86d393ca64f96 |
| رقم الانضمام: | edsbas.E07E410D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 09701591 19983824 |
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| DOI: | 10.4103/0970-1591.139570 |