Academic Journal
Effect of dichlorvos on hepatic and pancreatic glucokinase activity and gene expression, and on insulin mRNA levels.
| العنوان: | Effect of dichlorvos on hepatic and pancreatic glucokinase activity and gene expression, and on insulin mRNA levels. |
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| المؤلفون: | JOSE GUILLERMO ROMERO NAVARRO, TERESITA GUADALUPE LOPEZ ACEVES, ALBERTO ROJAS OCHOA, MARIA CRISTINA REGINA FERNANDEZ MEJIA |
| المصدر: | Life Sciences 78(9):1015 - 1020 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | info:eu-repo/classification/cti/3, Diclorvos - Farmacología - Ratas, Regulación de la expresión génica - Genética - Ratas, Enzimológica -Efectos de drogas - Ratas, Glucoquinasa - Metabolismo - Ratas, Insecticidas - Farmacología - Ratas, Insulina - Biosíntesis - Ratas, Dosificación letal mediana - Ratas, Hígado - Efectos de drogas - Ratas, Hígado - Enzimología - Ratas, Páncreas - Efectos de drogas - Ratas, Páncreas - Enzimología - Ratas, ARN Mensajero - Biosíntesis - Ratas, Ratas wistar, Dichlorvos - Pharmacology - Rats, Gene expression regulation - Genetics - Rats, Enzymologic -Drug effects - rats, Glucokinase - Metabolism - Rats, Insecticides - Pharmacology - Rats, Insulin - Biosynthesis - Rats, Lethal dose 50 - Rats, Liver - Drug effects - Rats, Liver - Enzymology - rats, Pancreas - Drug effects - Rats, Pancreas - Enzymology - Rats, RNA, messenger -Biosynthesis - Rats, Rats, wistar, Organofosforados |
| الوصف: | Several studies have shown that organophosphate pesticides affect carbohydrate metabolism and produce hyperglycemia. It has been reported that exposure to the organophosphate pesticide dichlorvos affects glucose homeostasis and decreases liver glycogen content. Glucokinase (EC 2.7.1.1) is a tissue-specific enzyme expressed in liver and in pancreatic beta cells that plays a crucial role in glycogen synthesis and glucose homeostasis. In the present study we analyzed the effect of one or three days of dichlorvos administration [20 mg/kg body weight] on the activity and mRNA levels of hepatic and pancreatic glucokinase as well as on insulin mRNA abundance in the rat. We found that the pesticide affects pancreatic and hepatic glucokinase activity and expression differently. In the liver the pesticide decreased the enzyme activity; on the contrary glucokinase mRNA levels were increased. In contrast, pancreatic glucokinase activity as well as mRNA levels were not affected by the treatment. Insulin mRNA levels were not modified by dichlorvos administration. Our results suggest that the decreased activity of hepatic glucokinase may account for the adverse effects of dichlorvos on glucose metabolism. © 2005 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3119 |
| DOI: | 10.1016/j.lfs.2005.06.010 |
| الاتاحة: | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3119 https://hdl.handle.net/20.500.12103/3119 https://doi.org/10.1016/j.lfs.2005.06.010 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0 |
| رقم الانضمام: | edsbas.E00344B5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.lfs.2005.06.010 |
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