Academic Journal
Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry
| العنوان: | Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry |
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| المؤلفون: | Fontanarosa, Carolina, PANE, FRANCESCA, Sepe, N., Pinto, G., TRIFUOGGI, MARCO, Squillace, M., ERRICO, FRANCESCO, Usiello, A., PUCCI, PIETRO, AMORESANO, ANGELA |
| المساهمون: | Fontanarosa, Carolina, Pane, Francesca, Sepe, N., Pinto, G., Trifuoggi, Marco, Squillace, M., Errico, Francesco, Usiello, A., Pucci, Pietro, Amoresano, Angela |
| سنة النشر: | 2017 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| الوصف: | Several studies have suggested that free D-Asp has a crucial role in N-methyl D-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determination of free D-Asp, L-Asp and N-methyl D-Asp in specimens of different mouse brain tissues using chiral LC-MS/MS in Multiple Reaction Monitoring scan mode. After comparing three procedures and different buffers and extraction solvents, a simple preparation procedure was selected the analytes of extraction. The method was validated by analyzing L-Asp, D-Asp and N-methyl D-Asp recovery at different spiked concentrations (50, 100 and 200 pg/μl) yielding satisfactory recoveries (75-110%), and good repeatability. Limits of detection (LOD) resulted to be 0.52 pg/μl for D-Asp, 0.46 pg/μl for L-Asp and 0.54 pg/μl for NMDA, respectively. Limits of quantification (LOQ) were 1.57 pg/μl for D-Asp, 1.41 pg/μl for L-Asp and 1.64 pg/μl for NMDA, respectively. Different concentration levels were used for constructing the calibration curves which showed good linearity. The validated method was then successfully applied to the simultaneous detection of D-Asp, L-Asp and NMDA in mouse brain tissues. The concurrent, sensitive, fast, and reproducible measurement of these metabolites in brain tissues will be useful to correlate the amount of free D-Asp with relevant neurological processes, making the LC-MS/MS MRM method well suited, not only for research work but also for clinical analyses. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000404608300046; volume:12; issue:6; firstpage:1; lastpage:17; numberofpages:17; journal:PLOS ONE; http://hdl.handle.net/11588/681242; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85021647915 |
| DOI: | 10.1371/journal.pone.0179748 |
| الاتاحة: | http://hdl.handle.net/11588/681242 https://doi.org/10.1371/journal.pone.0179748 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DFFBD16F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0179748 |
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