Academic Journal
Plasma phospholipid transfer protein prevents vascular endothelium dysfunction by delivering α-tocopherol to endothelial cells
| العنوان: | Plasma phospholipid transfer protein prevents vascular endothelium dysfunction by delivering α-tocopherol to endothelial cells |
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| المؤلفون: | Desrumaux, Catherine, M, Deckert, Valérie, Athias, Anne, Masson, David, Lizard, Gérard, Palleau, Viviane, Gambert, Philippe, Lagrost, Laurent |
| المساهمون: | Laboratoire des Lipoprotéines humaines et interactions vasculaires, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital du Bocage, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon) |
| المصدر: | ISSN: 0892-6638. |
| بيانات النشر: | HAL CCSD Federation of American Society of Experimental Biology |
| سنة النشر: | 1999 |
| المجموعة: | Université de Bourgogne (UB): HAL |
| مصطلحات موضوعية: | Acetylcholine, Aorta, Lipid transfer protein, Oxidized LDL, Vitamin E, MESH: Animals, MESH: Aorta, Thoracic / drug effects, MESH: Phospholipids / blood, MESH: Rabbits, Thoracic / physiology, MESH: Receptors, LDL / metabolism, MESH: Vasodilation / drug effects, MESH: Vitamin E / metabolism, MESH: Vitamin E / pharmacology, MESH: Biological Transport, Active / drug effects, MESH: Carrier Proteins / blood, MESH: Carrier Proteins / pharmacology, MESH: Humans, MESH: Cell Line, MESH: Endothelium, Vascular / cytology, Vascular / metabolism, MESH: Female, MESH: In Vitro Techniques, MESH: Lipoproteins, HDL / metabolism, MESH: Male |
| الوصف: | International audience ; α-tocopherol, the most potent antioxidant form of vitamin E, is mainly bound to lipoproteins in plasma and its incorporation into the vascular wall can prevent the endothelium dysfunction at an early stage of atherogenesis. In the present study, the plasma phospholipid transfer protein (PLTP) was shown to promote the net mass transfer of α-tocopherol from high density lipoproteins (HDL) and α-tocopherol-albumin complexes toward α-tocopherol-depleted, oxidized low density lipoproteins (LDL). The facilitated transfer reaction of α-tocopherol could be blocked by specific anti-PLTP antibodies. These observations indicate that PLTP may restore the antioxidant potential of plasma LDL at an early stage of the oxidation cascade that subsequently leads to cellular damages. In addition, the present study demonstrated that the PLTP-mediated net mass transfer of α-tocopherol can constitute a new mechanism for the incorporation of α-tocopherol into the vascular wall in addition to the previously recognized LDL receptor and lipoprotein lipase pathways. In ex vivo studies on rabbit aortic segments, the impairment of the endothelium-dependent arterial relaxation induced by oxidized LDL was found to be counteracted by a pretreatment with purified PLTP and α-tocopherol-albumin complexes, and both the maximal response and the sensitivity to acetylcholine were significantly improved. We conclude that PLTP, by supplying oxidized LDL and endothelial cells with α-tocopherol through a net mass transfer reaction may play at least two distinct beneficial roles in preventing endothelium damage, i.e., the antioxidant protection of LDL and the preservation of a normal relaxing function of vascular endothelial cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04207349; https://hal.science/hal-04207349; https://hal.science/hal-04207349/document; https://hal.science/hal-04207349/file/The%20FASEB%20Journal%20-%201999%20-%20Desrumaux.pdf |
| DOI: | 10.1096/fasebj.13.8.883 |
| الاتاحة: | https://hal.science/hal-04207349 https://hal.science/hal-04207349/document https://hal.science/hal-04207349/file/The%20FASEB%20Journal%20-%201999%20-%20Desrumaux.pdf https://doi.org/10.1096/fasebj.13.8.883 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.DF8CBC0C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1096/fasebj.13.8.883 |
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