Academic Journal
The Conformation of Yeast Chromosome III Is Mating Type Dependent and Controlled by the Recombination Enhancer
| العنوان: | The Conformation of Yeast Chromosome III Is Mating Type Dependent and Controlled by the Recombination Enhancer |
|---|---|
| المؤلفون: | Belton, Jon-Matthew, Lajoie, Bryan R., Audibert, Sylvain, Cantaloube, Sylvain, Lassadi, Imen, Goiffon, Isabelle, Bau, Davide, Marti-Renom, Marc A., Bystricky, Kerstin, Dekker, Job |
| المساهمون: | Department of Biochemistry and Molecular Pharmacology, Program in Systems Biology |
| المصدر: | Cell reports ; 13 ; 9 ; 1855-67 |
| سنة النشر: | 2022 |
| المجموعة: | University of Massachusetts, Medical School: eScholarship@UMMS |
| مصطلحات موضوعية: | chromosome conformation, long-range interactions, mating-type switching, multicolor fluorescence microscopy, recombination enhancer, Bioinformatics, Computational Biology, Genomics, Structural Biology, Systems Biology |
| الوصف: | Mating-type switching in yeast occurs through gene conversion between the MAT locus and one of two silent loci (HML or HMR) on opposite ends of the chromosome. MATa cells choose HML as template, whereas MATalpha cells use HMR. The recombination enhancer (RE) located on the left arm regulates this process. One long-standing hypothesis is that switching is guided by mating-type-specific and possibly RE-dependent chromosome folding. Here, we use Hi-C, 5C, and live-cell imaging to characterize the conformation of chromosome III in both mating types. We discovered a mating-type-specific conformational difference in the left arm. Deletion of a 1-kb subregion within the RE, which is not necessary during switching, abolished mating-type-dependent chromosome folding. The RE is therefore a composite element with one subregion essential for donor selection during switching and a separate region involved in modulating chromosome conformation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Link to Article in PubMed; Cell Rep. 2015 Dec 1;13(9):1855-67. doi:10.1016/j.celrep.2015.10.063. Epub 2015 Nov 19. Link to article on publisher's site; 2211-1247 (Electronic); http://hdl.handle.net/20.500.14038/39850; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3653&context=oapubs&unstamped=1; https://escholarship.umassmed.edu/oapubs/2649; 7972029; oapubs/2649 |
| DOI: | 10.1016/j.celrep.2015.10.063 |
| الاتاحة: | https://doi.org/10.1016/j.celrep.2015.10.063 https://hdl.handle.net/20.500.14038/39850 https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3653&context=oapubs&unstamped=1 https://escholarship.umassmed.edu/oapubs/2649 |
| Rights: | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.DF0956D8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2015.10.063 |
|---|