Academic Journal
Method development for detection of E545A mutation PIK3CA gene in breast cancer patients using Tm Shift SYBR Green I qPCR
| العنوان: | Method development for detection of E545A mutation PIK3CA gene in breast cancer patients using Tm Shift SYBR Green I qPCR |
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| المؤلفون: | Al Ahwani, Fuad, Desriani, Desriani, Widyastuti, Utut, Suharsono, Suharsono |
| المصدر: | Indonesian Journal of Biotechnology; Vol 21, No 1 (2016); 22-28 ; 2089-2241 ; 0853-8654 |
| بيانات النشر: | Universitas Gadjah Mada |
| سنة النشر: | 2016 |
| المجموعة: | Universitas Gadjah Mada Online Journals |
| مصطلحات موضوعية: | E545A, breast cancer, Ct, PIK3CA, precision, Tm |
| الوصف: | E545A is one of the point mutations, and its frequency is high in PIK3CA gene (3.8%), particularly breast cancer patients in Singapore (13.8%) and Mexico (11.5%). In addition to induce breast cancer, the mutation also caused resistance of anti-HER2 in HER2 cancer subtype. The tremendous effect of this mutation was not supported by affordable detection method. This study aimed to develop a feasible and sensitive method of E545A detection. The developing method used Tm and Ct to identify samples. Based on optimization, the best condition was obtained at optimization 2 at annealing temperature of 65°C. At this condition, Tm and Ct of each sample were (a) exon 9 (78.4°C and 13.005±0.007) and (b) E5454A (80.4°C and 10.07±0.1). This method also demonstrated good precision as observed in variance coefficient of intra and inter assay (0). Thus, method for E5454A detection mutation was successfully developed. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://jurnal.ugm.ac.id/ijbiotech/article/view/26815/16537; https://jurnal.ugm.ac.id/ijbiotech/article/view/26815 |
| DOI: | 10.22146/ijbiotech.26815 |
| الاتاحة: | https://jurnal.ugm.ac.id/ijbiotech/article/view/26815 https://doi.org/10.22146/ijbiotech.26815 |
| Rights: | Copyright (c) 2016 The Author(s) ; http://creativecommons.org/licenses/by-sa/4.0 |
| رقم الانضمام: | edsbas.DEF68E50 |
| قاعدة البيانات: | BASE |
| DOI: | 10.22146/ijbiotech.26815 |
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