Academic Journal
Dynamics of a Sporadic Nosocomial Acinetobacter calcoaceticus - Acinetobacter baumannii Complex Population
| العنوان: | Dynamics of a Sporadic Nosocomial Acinetobacter calcoaceticus - Acinetobacter baumannii Complex Population |
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| المؤلفون: | Villalon-Panzano, Pilar, Ortega, Montserrat, Saez-Nieto, Juan Antonio, Carrasco, Gema, Medina-Pascual, Maria Jose, Garrido, Noelia, Valdezate, Sylvia |
| بيانات النشر: | Frontiers Media |
| سنة النشر: | 2019 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | Acinetobacter calcoaceticus-baumannii complex, Antimicrobial susceptibility, Clonal distribution, Epidemic strains, Species identification, Sporadic strains |
| الوصف: | Our objective was to improve current knowledge of sporadic (Spo) nosocomial Acinetobactercalcoaceticus-Acinetobacter baumannii (Acb) complex populations, and thus better understand the epidemiology of Spo and endemoepidemic (EE) strains. Between 1999 and 2010, 133 isolates of Spo Acb complex were obtained from a single hospital. Species were identified by gyrB-PCR, and via gyrB- and rpoB-sequencing. Clonal analysis was undertaken using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Susceptibility to antimicrobial agents was determined by microdilution and E-tests. Carbapenemase genes were detected by PCR. One hundred and one PFGE types were detected. A. baumannii was the most common (67/101 PFGE types), followed by Acinetobacter pittii (22/101), Acinetobacter lactucae (6/101), and Acinetobacter calcoaceticus (2/101). gyrB, rpoB1, and rpoB2 sequencing returned 49, 13, and 16 novel sequences, respectively. Sixty-three sequence types (STs) (38 new STs and 66 new alleles) were detected; the most common were ST2 (29/133 isolates) and ST132 (14/133). Twenty-six OXA-51 allelic variants were detected, nine of which were novel. The PFGE types were generally susceptible (88/101) to all the tested antimicrobials; 3/101 were carbapenem-resistant due to the presence of the genetic structure ISAba2-blaOXA-58-like-ISAba3, and 2/101 were multidrug-resistant. It can be concluded that the examined Spo Acb complex population was mainly composed of A. baumannii. Many different clones were detected (with ST2 clearly dominant), all largely susceptible to antimicrobials; multidrug resistance was rare. In contrast, a previously examined EE Acb population was composed of just four expanding, multidrug-resistant A. baumannii clones -ST2, ST3, ST15, and ST80-. ; We thank Dr. Teresa Cabezas for the isolation and submission of the Acinetobacter spp. strains to the Reference and Research Laboratory for Taxonomy at the CNM, and Adrian Burton for editing and language assistance (Physical Evidence Scientific ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1664-302X 30967856 |
| Relation: | https://doi.org/10.3389/fmicb.2019.00593; Front Microbiol. 2019 Mar 22;10:593.; http://hdl.handle.net/20.500.12105/9120; Frontiers in microbiology |
| DOI: | 10.3389/fmicb.2019.00593 |
| الاتاحة: | https://hdl.handle.net/20.500.12105/9120 https://doi.org/10.3389/fmicb.2019.00593 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; Atribución 4.0 Internacional ; open access |
| رقم الانضمام: | edsbas.DEB2AA8B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664302X 30967856 |
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| DOI: | 10.3389/fmicb.2019.00593 |