Academic Journal
Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids
| العنوان: | Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids |
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| المؤلفون: | Kumar Gupta, Ashwani, Sarkar, Prasenjit, Wertheim, Jason A, Pan, Xinchao, Carroll, Thomas J, Oxburgh, Leif |
| المصدر: | Maine Medical Center |
| بيانات النشر: | MaineHealth Knowledge Connection |
| سنة النشر: | 2020 |
| المجموعة: | MaineHealth Knowledge Connection |
| مصطلحات موضوعية: | Animals, Cell Differentiation, Cell Line, Female, Humans, Kidney (growth & development), Male, Mice, Organogenesis (physiology), Organoids (growth & development), Pluripotent Stem Cells (transplantation) |
| الوصف: | A fundamental challenge in emulating kidney tissue formation through directed differentiation of human pluripotent stem cells is that kidney development is iterative, and to reproduce the asynchronous mix of differentiation states found in the fetal kidney we combined cells differentiated at different times in the same organoid. Asynchronous mixing promoted nephrogenesis, and heterochronic organoids were well vascularized when engrafted under the kidney capsule. Micro-CT and injection of a circulating vascular marker demonstrated that engrafted kidney tissue was connected to the systemic circulation by 2 weeks after engraftment. Proximal tubule glucose uptake was confirmed, but despite these promising measures of graft function, overgrowth of stromal cells prevented long-term study. We propose that this is a technical feature of the engraftment procedure rather than a specific shortcoming of the directed differentiation because kidney organoids derived from primary cells and whole embryonic kidneys develop similar stromal overgrowth when engrafted under the kidney capsule. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| Relation: | https://knowledgeconnection.mainehealth.org/mmc/3050; https://pubmed.ncbi.nlm.nih.gov/32393756/ |
| الاتاحة: | https://knowledgeconnection.mainehealth.org/mmc/3050 https://pubmed.ncbi.nlm.nih.gov/32393756/ |
| رقم الانضمام: | edsbas.DEA6ACD3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |