التفاصيل البيبلوغرافية
| العنوان: |
The Role of Cytochrome P450 AbyV in the Final Stages of Abyssomicin C Biosynthesis |
| المؤلفون: |
Devine AJ, Parnell AE, Back CR, Lees NR, Johns ST, Zulkepli AZ, Barringer R, Zorn K, Stach JEM, Crump MP, Hayes MA, van der Kamp MW, Race PR, Willis CL |
| المصدر: |
Angewandte Chemie - International Edition, 2022 |
| بيانات النشر: |
John Wiley and Sons Inc |
| سنة النشر: |
2022 |
| المجموعة: |
Newcastle University Library ePrints Service |
| الوصف: |
© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.Abyssomicin C and its atropisomer are potent inhibitors of bacterial folate metabolism. They possess complex polycyclic structures, and their biosynthesis has been shown to involve several unusual enzymatic transformations. Using a combination of synthesis and in vitro assays we reveal that AbyV, a cytochrome P450 enzyme from the aby gene cluster, catalyses a key late-stage epoxidation required for the installation of the characteristic ether-bridged core of abyssomicin C. The X-ray crystal structure of AbyV has been determined, which in combination with molecular dynamics simulations provides a structural framework for our functional data. This work demonstrates the power of combining selective carbon-13 labelling with NMR spectroscopy as a sensitive tool to interrogate enzyme-catalysed reactions in vitro with no need for purification. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| Relation: |
https://eprints.ncl.ac.uk/288305; https://eprints.ncl.ac.uk/fulltext.aspx?url=288305/7389733D-A088-4608-B42A-7FC7640A7B1C.pdf&pub_id=288305 |
| الاتاحة: |
https://eprints.ncl.ac.uk/288305 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.DE92C79D |
| قاعدة البيانات: |
BASE |