Academic Journal
A qPCR to quantify Wolbachia from few Onchocerca volvulus microfilariae as a surrogate for adult worm histology in clinical trials of antiwolbachial drugs
| العنوان: | A qPCR to quantify Wolbachia from few Onchocerca volvulus microfilariae as a surrogate for adult worm histology in clinical trials of antiwolbachial drugs |
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| المؤلفون: | Schlabe, Stefan, Korir, Patricia, Lämmer, Christine, Landmann, Frederic, Dubben, Bettina, Koschel, Marianne, Albers, Anna, Debrah, Linda Batsa, Debrah, Alexander Yaw, Hübner, Marc, Pfarr, Kenneth, Klarmann-Schulz, Ute, Hoerauf, Achim |
| المساهمون: | German Center for Infection Research - Partner Site Bonn-Cologne (DZIF), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Kwame Nkrumah University of Science and Technology (KNUST) |
| المصدر: | ISSN: 0932-0113. |
| بيانات النشر: | HAL CCSD Springer Verlag (Germany) |
| سنة النشر: | 2022 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | qPCR, Wolbachia, Onchocerca volvulus, Litomosoides sigmodontis, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| الوصف: | International audience ; Abstract The filarial nematode Onchocerca volvulus causes onchocerciasis (river blindness), a neglected tropical disease affecting 21 million people, mostly in Sub-Saharan Africa. Targeting the endosymbiont Wolbachia with antibiotics leads to permanent sterilization and killing of adult worms. The gold standard to assess Wolbachia depletion is the histological examination of adult worms in nodules beginning at 6 months post-treatment. However, nodules can only be used once, limiting the time points to monitor Wolbachia depletion. A diagnostic to longitudinally monitor Wolbachia depletion from microfilariae (MF) at more frequent intervals < 6 months post-treatment would accelerate clinical trials of antiwolbachials. We developed a TaqMan qPCR amplifying the single-copy gene w Ov ftsZ to quantify Wolbachia from as few as one MF that had migrated from skin biopsies and compared quantification using circular and linearized plasmids or synthetic dsDNA (gBlock®). qPCR for MF from the rodent nematode Litomosoides sigmodontis was used to support the reproducibility and validate the principle. The qPCR using as few as 2 MF from O. volvulus and L. sigmodontis reproducibly quantified Wolbachia . Use of a linearized plasmid standard or synthesized dsDNA resulted in numbers of Wolbachia /MF congruent with biologically plausible estimates in O. volvulus and L. sigmodontis MF. The qPCR assay yielded a median of 48.8 (range 1.5–280.5) Wolbachia / O. volvulus MF. The qPCR is a sensitive tool for quantifying Wolbachia in a few MF from skin biopsies and allows for establishing the qPCR as a surrogate parameter for monitoring Wolbachia depletion in adult worms of new antiwolbachial candidates. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/s00436-021-07411-5 |
| الاتاحة: | https://cnrs.hal.science/hal-03813246 https://cnrs.hal.science/hal-03813246v1/document https://cnrs.hal.science/hal-03813246v1/file/436_2021_Article_7411.pdf https://doi.org/10.1007/s00436-021-07411-5 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.DE8EBA58 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00436-021-07411-5 |
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