Conference
Energy Requirement for Maintenance of Adenylate Energy Charge and Metabolic ATP in Platelets During Starvation
| العنوان: | Energy Requirement for Maintenance of Adenylate Energy Charge and Metabolic ATP in Platelets During Starvation |
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| المؤلفون: | Akkerman, J.W.N., Gorter, G., Sixma, J.J. |
| المصدر: | VIth International Congress on Thrombosis and Haemostasis ; Thrombosis and Haemostasis ; ISSN 2567-689X |
| بيانات النشر: | Schattauer GmbH |
| سنة النشر: | 1977 |
| الوصف: | Energy requirements for maintenance of stable adenylate energy charge (AEC) and metabolic ATP(ATP-m)level were studied in gel filtered platelets at various degrees of starvation. Platelets gel filtered and subsequently incubated during 40 min.at 37°C with 1mM CN- and without glucose consumed their glycogen at a rate of 0.79 ± 0.23(± SD, n=6)/μmol glycosyl residues .min-1 10-11 cells. During this period AEC and ATP-m decreased linearly with time at rates of 5-6.10-3 and 0.75-1.05% of total radioactive adenine nucleotides .min-1.10-11 cells respectively. Addition of 25–1000μM glucose increased lactate production and decreased the fall of AEC and ATP-m proportional to the amounts of glucose added. Glycogenolysis remained active below 100μM glucose but ceased at higher glucose concentrations. From these data ATP-m production from glycogenolysis and glycolysis was calculated and compared with the decrease of steady state levels of AEC and ATP-m. A production of 3μmol ATP-m.min-1.10-11 cells was required to maintain initial AEC and ATP-m level. At lower rates of ATP-m production these values fell without reaching stable steady state levels in a lower range. After 40-50 min variations in AEC and ATP-m ceased and lactate formation stopped leaving the cells in a state of hybernation. Subsequent addition of glucoserestored lactate accumulation, AEC and ATP-m. On the basis of formation and steady state levels of ATP-m its consumption was calculated. A lowering production was not completely met by a lowering consumption. Energy consumption in resting platelets is therefore partly independent from energy production. |
| نوع الوثيقة: | conference object |
| اللغة: | unknown |
| DOI: | 10.1055/s-0039-1680639 |
| DOI: | 10.1055/s-0039-1680639.pdf |
| الاتاحة: | http://dx.doi.org/10.1055/s-0039-1680639 http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0039-1680639.pdf |
| رقم الانضمام: | edsbas.DE72FC00 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1055/s-0039-1680639 |
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