Academic Journal
Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles
| العنوان: | Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles |
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| المؤلفون: | Corchete Sánchez, María Purificación, Almagro, Lorena, Gabaldón, Jose Antonio, Pedreño, María Angeles, Palazón, Javier |
| بيانات النشر: | Springer |
| سنة النشر: | 2022 |
| المجموعة: | Universidad de Salamanca: Gredos (Gestión del Repositorio Documental de la Universidad de Salamanca) |
| مصطلحات موضوعية: | Silybum marianum suspension cultures, Hydroxypropyl-β-cyclodextrin polymer-coated magnetic nanoparticles, Methyl jasmonate, t-resveratrol, Naringenin, 2417 Biología Vegetal (Botánica), 2407.01 Cultivo Celular |
| الوصف: | [EN] The glucose oligosaccharide-derived cyclodextrins (CDs) are used for improving bioactive compound production in plant cell cultures because, in addition to their elicitation activity, CDs promote product removal from cells. However, despite these advantages, the industrial application of CDs is hampered by their high market price. A strategy to overcome this constraint was recently tested, in which reusable CD polymers coated with magnetic Fe3O4 nanoparticles were harnessed in Vitis vinifera cell cultures to produce t-resveratrol (t-R). In this study, we applied hydroxypropyl-β-CDs (HPCD) and HPCDs coated with magnetic nanoparticles (HPCD-EPI-MN) in methyl jasmonate (MJ)-treated transgenic Silybum marianum cultures ectopically expressing either a stilbene synthase gene (STS) or a chalcone synthase gene (CHS), and compared their efects on the yields of t-R and naringenin (Ng), respectively. HPCD-EPI-MN at 15 g/L stimulated the accumulation of metabolites in the culture medium of the corresponding transgenic cell lines, with up to 4 mg/L of t-R and 3 mg/L of Ng released after 3 days. Similar amounts were produced in cultures treated with HPCD. Concentrations higher than 15 g/L of HPCD-EPI-MN and prolonged incubation periods negatively afected cell growth and viability in both transgenic cell lines. Reutilization of HPCD-EPI-MN was possible in three elicitation cycles (72 h each), after which the polymer retained 25–30% of its initial efciency, indicating good stability and reusability. Due to their capacity to adsorb metabolites and their recyclability, the application of magnetic CD polymers may reduce the costs of establishing efcient secondary metabolite production systems on a commercial scale. ; Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLE. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0175-7598 1432-0614 |
| Relation: | https://doi.org/10.1007/s00253-022-11886-2; Corchete, P., Almagro, L., Gabaldón, J.A. et al (2022). Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles. Appl Microbiol Biotechnol 106, 2393–2401. https://doi.org/10.1007/s00253-022-11886-2; http://hdl.handle.net/10366/150935 |
| DOI: | 10.1007/s00253-022-11886-2 |
| الاتاحة: | http://hdl.handle.net/10366/150935 https://doi.org/10.1007/s00253-022-11886-2 |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DE55FC40 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 01757598 14320614 |
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| DOI: | 10.1007/s00253-022-11886-2 |