التفاصيل البيبلوغرافية
| العنوان: |
OVA/DOTAP/D35/Alhydrogel immunization induces strong T cell and antibody responses. |
| المؤلفون: |
Yasunari Haseda (8356929), Lisa Munakata (8356932), Chiyo Kimura (11218258), Yumi Kinugasa-Katayama (10968546), Yasuko Mori (668955), Ryo Suzuki (138376), Taiki Aoshi (392924) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Cell Biology, Molecular Biology, Biotechnology, Immunology, Cancer, Hematology, Infectious Diseases, Space Science, antibody response induction, combination adjuvant, model protein antigen, combination adjuvant formulation, T cell responses, antigen uptake DC migration, component, DOTAP, novel combination adjuvant, T cell, protein antigens need, vaccine adjuvant candidate, model antigen interaction studies |
| الوصف: |
C57BL/6J mice were immunized i.d. with OVA and the indicated adjuvant combinations at the tail base. OVA (10 μg), DOTAP (100 μg), D35 (10 μg), and Alhydrogel (40 μg) were mixed in a 1:1 mixture of 5% glucose and phosphate-buffered saline (PBS)(Glu/PBS buffer). After 7 days of immunization, splenocytes and serum were collected. The splenocytes were stimulated in vitro with OVA257-264 peptide that induces MHC class I restricted response (CD8 + T cell response), OVA323-339 peptide, or OVA protein that induces MHC class II restricted response (CD4 + T cell response). (a) After 24 h, the concentration of mouse IFN-γ in the culture supernatant was measured by ELISA. (b) The OVA-specific antibody titers of total IgG, IgG1, and IgG2c were determined by ELISA after 7 days of the single immunization. The bar graph indicates the mean + SD of cytokine concentration observed in six mice per group (a) and antibody titer calculated from three mice per group (b). Statistical significance compared with OVA only is shown by asterisks in the bar graph; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/OVA_DOTAP_D35_Alhydrogel_immunization_induces_strong_T_cell_and_antibody_responses_/15091552 |
| DOI: |
10.1371/journal.pone.0254628.g001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0254628.g001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.DE36FF77 |
| قاعدة البيانات: |
BASE |