Academic Journal
Trans-dissemination of exosomes from HIV-1-infected cells fosters both HIV-1 trans-infection in resting CD4+T lymphocytes and reactivation of the HIV-1 reservoir
| العنوان: | Trans-dissemination of exosomes from HIV-1-infected cells fosters both HIV-1 trans-infection in resting CD4+T lymphocytes and reactivation of the HIV-1 reservoir |
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| المؤلفون: | Chiozzini, Chiara, Arenaccio, Claudia, Olivetta, Eleonora, Anticoli, Simona, Manfredi, Francesco, Ferrantelli, Flavia, d’Ettorre, Gabriella, Schietroma, Ivan, ANDREOTTI, MAURO, Federico, Maurizio |
| المساهمون: | Chiozzini, Chiara, Arenaccio, Claudia, Olivetta, Eleonora, Anticoli, Simona, Manfredi, Francesco, Ferrantelli, Flavia, D’Ettorre, Gabriella, Schietroma, Ivan, Andreotti, Mauro, Federico, Maurizio |
| بيانات النشر: | Springer-Verlag Wien Vienna |
| سنة النشر: | 2017 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | GPR37 Cell, GW4869, iodixanol, virological synapse, cART patient, adult, anti-HIV agent, CD4-positive T-Lymphocyte, cell Line, coculture technique, drug therapy, combination, exosome, HIV infection, HIV-1, human, male, virus activation, virology |
| الوصف: | Intact HIV-1 and exosomes can be internalized by dendritic cells (DCs) through a common pathway leading to their transmission to CD4? T lymphocytes by means of mechanisms defined as trans-infection and trans- dissemination, respectively. We previously reported that exosomes from HIV-1-infected cells activate both unin- fected quiescent CD4? T lymphocytes, which become permissive to HIV-1, and latently infected cells, with release of HIV-1 particles. However, nothing is known about the effects of trans-dissemination of exosomes pro- duced by HIV-1-infected cells on uninfected or latently HIV-1-infected CD4? T lymphocytes. Here, we report that trans-dissemination of exosomes from HIV-1-infected cells induces cell activation in resting CD4? T lympho- cytes, which appears stronger with mature than immature DCs. Using purified preparations of both HIV-1 and exo- somes, we observed that mDC-mediated trans-dissemina- tion of exosomes from HIV-1-infected cells to resting CD4? T lymphocytes induces efficient trans-infection and HIV-1 expression in target cells. Most relevant, when both mDCs and CD4? T lymphocytes were isolated from combination anti-retroviral therapy (ART)-treated HIV-1- infected patients, trans-dissemination of exosomes from HIV-1-infected cells led to HIV-1 reactivation from the viral reservoir. In sum, our data suggest a role of exosome trans-dissemination in both HIV-1 spread in the infected host and reactivation of the HIV-1 reservoir. & Maurizio Federico maurizio.federico@iss.it 1 Introduction HIV-1 can be delivered to uninfected target cells by den- dritic cells (DCs) through a process referred to as trans- infection [1, 2], which is mediated by the formation of virological synapses containing HIV-1-loaded DCs and target CD4? T lymphocytes [3, 4]. DCs support HIV-1 replication poorly, which is mostly a consequence of the intracellular expression of the Sam domain and HD domain-containing protein (SAMHD)-1 cell restriction factor, which acts by a twofold mechanism, i.e., by limiting ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28474225; info:eu-repo/semantics/altIdentifier/wos/WOS:000408059500003; volume:162; issue:9; firstpage:2565; lastpage:2577; numberofpages:13; journal:ARCHIVES OF VIROLOGY; http://hdl.handle.net/11573/1088006; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85018795124; link.springer.de/link/service/journals/00705 |
| DOI: | 10.1007/s00705-017-3391-4 |
| الاتاحة: | http://hdl.handle.net/11573/1088006 https://doi.org/10.1007/s00705-017-3391-4 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.DE2B053B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00705-017-3391-4 |
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