Academic Journal
Determining bacterial and host contributions to the human salivary metabolome
| العنوان: | Determining bacterial and host contributions to the human salivary metabolome |
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| المؤلفون: | Gardner, Alexander, Parkes, Harold G., So, Po Wah, Carpenter, Guy H. |
| المصدر: | Gardner , A , Parkes , H G , So , P W & Carpenter , G H 2019 , ' Determining bacterial and host contributions to the human salivary metabolome ' , Journal of Oral Microbiology , vol. 11 , no. 1 , 1617014 . https://doi.org/10.1080/20002297.2019.1617014 |
| سنة النشر: | 2019 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | metabolomics, NMR spectroscopy, oral microbiome, saliva, /dk/atira/pure/subjectarea/asjc/3500/3501, name=Dentistry (miscellaneous), /dk/atira/pure/subjectarea/asjc/2700/2726, name=Microbiology (medical), /dk/atira/pure/subjectarea/asjc/2700/2725, name=Infectious Diseases |
| الوصف: | Background : Salivary metabolomics is rapidly advancing. Aim and methods: To determine the extent to which salivary metabolites reflects host or microbial metabolic activity whole-mouth saliva (WMS), parotid saliva (PS) and plasma collected contemporaneously from healthy volunteers were analysed by 1 H-NMR spectroscopy. Spectra underwent principal component analysis and k-means cluster analysis and metabolite quantification. WMS samples were cultured on both sucrose and peptide-enriched media. Correlation between metabolite concentration and bacterial load was assessed. Results : WMS contained abundant short-chain fatty acids (SCFAs), which were minimal in PS and plasma. WMS spectral exhibited greater inter-individual variation than those of PS or plasma (6.7 and 3.6 fold, respectively), likely reflecting diversity of microbial metabolomes. WMS bacterial load correlated strongly with SCFA levels. Additional WMS metabolites including amines, amino acids and organic acids were positively correlated with bacterial load. Lactate, urea and citrate appeared to enter WMS via PS and the circulation. Urea correlated inversely with WMS bacterial load. Conclusions : Oral microbiota contribute significantly to the WMS metabolome. Several WMS metabolites (lactate, urea and citrate) are derived from the host circulation. WMS may be particularly useful to aid diagnosis of conditions reflective of dysbiosis. WMS could also complement other gastrointestinal fluids in future metabolomic studies. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://discovery.dundee.ac.uk/en/publications/d6549106-2bc3-4171-b52d-33f81da430ac |
| DOI: | 10.1080/20002297.2019.1617014 |
| الاتاحة: | https://discovery.dundee.ac.uk/en/publications/d6549106-2bc3-4171-b52d-33f81da430ac https://doi.org/10.1080/20002297.2019.1617014 https://discovery.dundee.ac.uk/ws/files/49103434/Determining_bacterial_and_host_contributions_to_the_human_salivary_metabolome.pdf http://www.scopus.com/inward/record.url?scp=85074126333&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DE2ADBFD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1080/20002297.2019.1617014 |
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