Academic Journal
Syk facilitates phagosome-lysosome fusion by regulating actin-remodeling in complement-mediated phagocytosis
| العنوان: | Syk facilitates phagosome-lysosome fusion by regulating actin-remodeling in complement-mediated phagocytosis |
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| المؤلفون: | Tabata, Hiroyuki, Morita, Hiroyuki, Kaji, Hiroaki, Tohyama, Kaoru, Tohyama, Yumi |
| المساهمون: | Grant-in-Aid for Scientific Research (KAKENHI) from Japan Society for the Promotion of Science, Grant program for biomedical engineering research from Nakatani Foundation |
| المصدر: | Scientific Reports ; volume 10, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2020 |
| الوصف: | Effective phagocytosis is crucial for host defense against pathogens. Macrophages entrap pathogens into a phagosome and subsequently acidic lysosomes fuse to the phagosome. Previous studies showed the pivotal role of actin-remodeling mediated by phosphoinositide-related signaling in phagosome formation, but the mechanisms of phagosome-lysosome fusion remain unexplored. Here we show that in complement-mediated phagocytosis, phagosome-lysosome fusion requires the disappearance of F-actin structure surrounding the phagosome and a tyrosine kinase Syk plays a key role in this process. Using macrophage-like differentiated HL60 and Syk-knockout (Syk-KO) HL60 cells, we found that Syk-KO cells showed insufficient phagosome acidification caused by impaired fusion with lysosomes and permitted the survival of Candida albicans in complement-mediated phagocytosis. Phagosome tracking analysis showed that during phagosome internalization process, F-actin surrounding phagosomes disappeared in both parental and Syk-KO cells but this structure was reconstructed immediately only in Syk-KO cells. In addition, F-actin-stabilizing agent induced a similar impairment of phagosome-lysosome fusion. Collectively, Syk-derived signaling facilitates phagosome-lysosome fusion by regulating actin-remodeling. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-020-79156-7 |
| الاتاحة: | http://dx.doi.org/10.1038/s41598-020-79156-7 https://www.nature.com/articles/s41598-020-79156-7.pdf https://www.nature.com/articles/s41598-020-79156-7 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.DE1BBEA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-020-79156-7 |
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