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A CG to NSC lineage interaction through Nrx-IV and Wrapper is required for individual encasing of NSC lineages by the CG.
| العنوان: | A CG to NSC lineage interaction through Nrx-IV and Wrapper is required for individual encasing of NSC lineages by the CG. |
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| المؤلفون: | Agata Banach-Latapy, Vincent Rincheval, David Briand, Isabelle Guénal, Pauline Spéder |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cell Biology, Neuroscience, Developmental Biology, Cancer, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, stem cell population, shapes adult behaviour, div >< p, defined cellular microenvironment, individual stem cell, related cells regardless, neural stem cells, altered cg network, individual encasing, also altered, wrapper exists, weaker interaction, stereotypic structure, resulting adults, nsc lineages, newborn neurons, neuroglian results, membranous chambers, mechanisms building, locomotor hyperactivity, larval brain, intralineage adhesion, functional relevance |
| الوصف: | ( A ) Representative confocal pictures at the NSC and neuropile levels of the extent of loss of signal for wrapper (monitored through the detection by RNA FISH of wrapper mRNA, magenta) under wrapper knockdown ( cyp > wrapper RNA BDSC29561 ) in CG (driver line Nrv2 :: GFP , tub-GAL80 ts ; cyp4g15-GAL4 ) compared to control ( cyp >— ( x w 1118 )). Larvae are dissected after 68 h at 29°C from ALH0. White arrows indicate wrapper mRNA signal coming from the midline glia. ( B ) Quantification of the efficiency of wrapper knockdown in NSC lineages by RNAi from ( A ). wrapper levels are monitored through RNA FISH. See Methods for details of the quantification. cyp >— ( x w 1118 ) ( n = 8 VNCs) and cyp > wrapper RNAi BDSC29561 ( n = 8 VNCs). Data statistics: unpaired Student t test. Results are presented as box and whisker plots. ( C ) Representative confocal pictures of the thoracic VNC for wrapper knockdown by RNAi ( cyp > wrapper RNA iVDRC105314 ) in the CG (driver line Nrv2 :: GFP , tub-GAL80 ts ; cyp4g15-GAL4 ). Larvae are dissected after 68 h at 29°C from ALH0. n = 8 VNCs. CG membrane is visualised by Nrv2 :: GFP (green), and NSCs are labelled with anti-Dpn (grey). ( D ) Representative confocal picture of the expression pattern of combined NSC lineages and CG drivers ( wor + cyp ) at the NSC level. UAS-mCD8 :: GFP and UAS-Histone2B :: RFP were both driven under the control of Nrv2 :: GFP , worniu-GAL4; cyp4g15-GAL4 , tub-GAL80 ts . Larvae are dissected after 68 h at 29°C from ALH0. n = 6 VNCs. GAL4 expression is visualised by the membrane staining of mCD8::GFP (magenta) and nuclear staining of Histone2B::RFP (magenta). NSCs are labelled with anti-Dpn (grey) and glia with anti-Repo (blue). Expression in the CG is detectable by the stereotypic membrane pattern, and co-localisation between Histone2B::RFP and Repo (white arrows). Expression in NSC lineages is detectable by the co-localisation between Histone2B::RFP and Dpn (yellow arrows) and the accumulation of RFP + progeny nuclei. ( E ) ... |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/A_CG_to_NSC_lineage_interaction_through_Nrx-IV_and_Wrapper_is_required_for_individual_encasing_of_NSC_lineages_by_the_CG_/24537865 |
| DOI: | 10.1371/journal.pbio.3002352.s007 |
| الاتاحة: | https://doi.org/10.1371/journal.pbio.3002352.s007 https://figshare.com/articles/figure/A_CG_to_NSC_lineage_interaction_through_Nrx-IV_and_Wrapper_is_required_for_individual_encasing_of_NSC_lineages_by_the_CG_/24537865 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.DE13549F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pbio.3002352.s007 |
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