التفاصيل البيبلوغرافية
| العنوان: |
Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico |
| المؤلفون: |
Cortés López, N. G., Sachman Ruiz, B., Montor Antonio, J. J., Miranda Sánchez, F., Alcántara Hernández, R. J., Del Moral, S. |
| المصدر: |
Nova scientia, ISSN 2007-0705, Vol. 10, Nº. 20, 2018, pags. 222-243 |
| سنة النشر: |
2018 |
| المجموعة: |
Dialnet - Portada de revistas (Universidad de La Rioja) |
| مصطلحات موضوعية: |
bacterial biodiversity, sugarcane, enriched culture, OTUs, biodiversidad bacteriana, caña de azúcar, cultivos enriquecidos, unidades taxonómicas operacionales |
| الوصف: |
Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing. Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch. Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter. ; Resumen: Introducción: La caña de azúcar es un producto agrícola esencial para producir bioetanol en México. El conocimiento de la comunidad bacteriana asociada a este cultivo y el estado del suelo es un paso decisivo para comprender cómo los microorganismos influyen en la productividad de los cultivos. Los cultivos enriquecidos con sustrato es una técnica que permite conocer la biodiversidad de muestras biológicas. El objetivo de esta investigación fue identificar a través de cultivos ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://dialnet.unirioja.es/servlet/oaiart?codigo=6938597 |
| الاتاحة: |
https://dialnet.unirioja.es/servlet/oaiart?codigo=6938597 |
| Rights: |
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| رقم الانضمام: |
edsbas.DD61C297 |
| قاعدة البيانات: |
BASE |