Academic Journal
Bacillus amyloliquefaciensas prophylactic treatment for Clostridium difficile‐associated disease in a mouse model
| العنوان: | Bacillus amyloliquefaciensas prophylactic treatment for Clostridium difficile‐associated disease in a mouse model |
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| المؤلفون: | Geeraerts, S, Ducatelle, R, Haesebrouck, F, Van Immerseel, F |
| المساهمون: | Institute for Promotion of Innovation |
| المصدر: | Journal of Gastroenterology and Hepatology ; volume 30, issue 8, page 1275-1280 ; ISSN 0815-9319 1440-1746 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background and Aim Probiotics might offer an attractive alternative for standard antibiotic therapy to treat C lostridium difficile infections ( CDI ). We specifically selected a B acillus amyloliquefaciens strain for its high in vitro antibacterial activity against C . difficile and tested its efficacy to prevent CDI in a mouse model. Methods B . amyloliquefaciens supernatant was tested against a large collection of C . difficile strains using an agar well diffusion test. B . amyloliquefaciens was orally administered to C57BL /6 mice in which CDI was induced using C . difficile VPI 10463, and its effect was compared with control mice receiving no treatment and mice receiving S accharomyces boulardii . Mice were followed up daily for signs of disease including weight loss. At necropsy, the colon was collected and subjected to histopathological analysis. C . difficile toxin A / B levels and colon weight/length and colon/body weight ratios were calculated. Results B . amyloliquefaciens supernatant was able to inhibit the growth of all C . difficile strains. Results of the in vivo trial indicated a significant weight loss for untreated and S . boulardii ‐treated mice as compared to B . amyloliquefaciens ‐treated mice. C . difficile toxin A and B levels were significantly higher for untreated and S . boulardii ‐treated mice than B . amyloliquefaciens ‐treated mice. A significantly lower degree of colon damage was detected for B . amyloliquefaciens ‐treated mice as compared to untreated and S . boulardii ‐treated mice, based on histopathological analysis, colon weight/length and colon/body weight ratios. Conclusion Administration of B . amyloliquefaciens was successful in preventing CDI in a mouse model. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jgh.12957 |
| الاتاحة: | http://dx.doi.org/10.1111/jgh.12957 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjgh.12957 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jgh.12957 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.DD5F8288 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jgh.12957 |
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