Academic Journal

Lupin (Lupinus spp.) seeds exert anthelmintic activity associated with their alkaloid content

التفاصيل البيبلوغرافية
العنوان: Lupin (Lupinus spp.) seeds exert anthelmintic activity associated with their alkaloid content
المؤلفون: Dubois, O., Allanic, Camille, Charvet, C., L, Guégnard, Fabrice, Février, H., Thery-Koné, Isabelle, Cortet, J., Koch, Christine, Bouvier, F., Fassier, T., Marcon, D., Magnin-Robert, J., B, Peineau, N., Courtot, E., Huau, C., Meynadier, Annabelle, Enguehard-Gueiffier, Cécile, Neveu, Cédric, Boudesocque-Delaye, Leslie, Sallé, Guillaume, Salle
المساهمون: Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Synthèse et isolement de molécules bio-actives (SIMBA), Université de Tours (UT), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Génétique Physiologie et Systèmes d'Elevage (GenPhySE), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT), Centre-Val de Loire region (Lupinema project, grant number 2014-00091669)
المصدر: ISSN: 2045-2322.
بيانات النشر: HAL CCSD
Nature Publishing Group
سنة النشر: 2019
المجموعة: Université François-Rabelais de Tours: HAL
مصطلحات موضوعية: [CHIM]Chemical Sciences, [SDV]Life Sciences [q-bio]
الوصف: Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-45654-6. ; International audience ; the growing range of drug resistant parasitic nematode populations threatens the sustainability of ruminant farming worldwide. In this context, nutraceuticals, animal feed that provides necessary dietary requirements while ensuring parasite control, could contribute to increase farming sustainability in developed and low resource settings. In this study, we evaluated the anthelmintic potential of lupin seed extracts against the major ruminant trichostrongylids, Haemonchus contortus and Teladorsagia circumcincta. In vitro observations showed that seed extracts from commercially available lupin varieties could significantly but moderately inhibit larval migration. This anthelmintic effect was mediated by the seed alkaloid content and was potent against both fully susceptible and multidrug resistant H. contortus isolates as well as a susceptible T. circumcincta isolate. Analytical chemistry revealed a set of four lupanine and sparteine-derivatives with anthelmintic activity, and electrophysiology assays on recombinant nematode acetylcholine receptors suggested an antagonistic mode of action for lupin alkaloids. An in vivo trial in H. contortus infected lupin-fed ewes and goats failed to demonstrate any direct anthelmintic effect of crude lupin seeds but infected lupin-fed goats suffered significantly less parasite-mediated blood losses. Altogether, our findings suggest that the anthelmintic potential of lupin remains limited. However, the potent alkaloids identified could lead to the development of novel drugs or may be used in combination with current anthelmintics to improve their efficacy.
نوع الوثيقة: article in journal/newspaper
اللغة: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/31227784; PRODINRA: 475598; PUBMED: 31227784; WOS: 000472478000008
DOI: 10.1038/s41598-019-45654-6
الاتاحة: https://hal.science/hal-02176665
https://hal.science/hal-02176665v1/document
https://hal.science/hal-02176665v1/file/2019-Scientific%20report%20Lupin.pdf
https://doi.org/10.1038/s41598-019-45654-6
Rights: http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
رقم الانضمام: edsbas.DD5BE7F4
قاعدة البيانات: BASE
الوصف
DOI:10.1038/s41598-019-45654-6