Academic Journal
A comparative analysis of miRNA expression in human lung epithelial cells during infection with influenza virus and RNAse treatment ; Сравнительный анализ экспрессии микроРНК в эпителиальных клетках легких человека при заражении вирусом гриппа и обработке РНКазой
| العنوان: | A comparative analysis of miRNA expression in human lung epithelial cells during infection with influenza virus and RNAse treatment ; Сравнительный анализ экспрессии микроРНК в эпителиальных клетках легких человека при заражении вирусом гриппа и обработке РНКазой |
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| المؤلفون: | Baichurina I.A., Markelova M.I., Shah Mahmud R. |
| المصدر: | Russian Journal of Infection and Immunity; Vol 12, No 2 (2022); 263-270 ; Инфекция и иммунитет; Vol 12, No 2 (2022); 263-270 ; 2313-7398 ; 2220-7619 |
| بيانات النشر: | SPb RAACI |
| سنة النشر: | 2022 |
| المجموعة: | Russian Journal of Infection and Immunity (Infektsiya i immunitet) / Инфекция и иммунитет |
| مصطلحات موضوعية: | microRNA, eukaryotic cells, influenza virus, cancer, biomarker, sequencing, микроРНК, клетки эукариот, вирус гриппа, рак, биомаркер, секвенирование |
| الوصف: | The influenza virus is capable of causing an acute respiratory infection that affects 5 to 20% of the human population annually. The spread of the influenza virus epidemic occurs within a short period of time due to its high contagiousness. In addition, the annual circulation of the virus among livestock and waterfowl increases for new strains a risk of zoonotic transmission to human populations with unestablished yet immunity. In addition, several high virulence pandemic strains have emerged in the past, and the threat of a new pandemic strain is constantly present. The identification of the physiological and molecular aspects related to influenza A can help developing therapeutic approaches to lower side effects associated with the disease caused by this virus. The RNA profile in human cells changes after exposure to influenza virus. Currently, scientists have been increasingly paying attention to study of microRNAs capable of regulating gene expression. Thus, microRNAs may play a critical role in a wide range of biological processes and have been previously shown to be important effectors in multilayered host-pathogen interplay. The study of the quantitative and qualitative miRNA composition is an important tool for diagnosing and treating various diseases at an early stage. The aim of this work is to analyze the microRNA profile for investigating an effect of influenza A (H1N1) virus on human lung epithelial adenocarcinoma cells. The microRNA fraction was isolated by using phenol-chloroform extraction and analyzed with high-throughput sequencing on the SOLiD 550xl wildfire platform using bioinformatic methods. The study examined 129 mature microRNAs from uninfected cells treated with Bacillus pumilus RNAse as well as cells infected with the influenza A (H1N1) virus. It was found that uninfected cells treated with RNase contained 2-fold more different microRNAs that can participate in suppressing carcinogenesis. The peak expression in influenza virus-infected cells is observed for miR-6884-5p. For cells ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Russian |
| Relation: | https://iimmun.ru/iimm/article/view/1454/1401; https://iimmun.ru/iimm/article/view/1454/1466; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5174; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5175; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5176; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5177; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5178; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5179; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5180; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5181; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5182; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5183; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5184; https://iimmun.ru/iimm/article/downloadSuppFile/1454/5207; https://iimmun.ru/iimm/article/downloadSuppFile/1454/6044; https://iimmun.ru/iimm/article/downloadSuppFile/1454/7567; https://iimmun.ru/iimm/article/downloadSuppFile/1454/7664; https://iimmun.ru/iimm/article/downloadSuppFile/1454/8104; https://iimmun.ru/iimm/article/downloadSuppFile/1454/8105; https://iimmun.ru/iimm/article/downloadSuppFile/1454/8106; https://iimmun.ru/iimm/article/downloadSuppFile/1454/8107; https://iimmun.ru/iimm/article/downloadSuppFile/1454/8108; https://iimmun.ru/iimm/article/downloadSuppFile/1454/8109; https://iimmun.ru/iimm/article/view/1454 |
| DOI: | 10.15789/2220-7619-ACA-1454 |
| الاتاحة: | https://iimmun.ru/iimm/article/view/1454 https://doi.org/10.15789/2220-7619-ACA-1454 |
| Rights: | Copyright (c) 2022 Baichurina I.A., Markelova M.I., Shah Mahmud R. ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.DD53455B |
| قاعدة البيانات: | BASE |
| DOI: | 10.15789/2220-7619-ACA-1454 |
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