Academic Journal

Taxonomical Evaluation of Plant Chloroplastic Markers by Bayesian Classifier

التفاصيل البيبلوغرافية
العنوان: Taxonomical Evaluation of Plant Chloroplastic Markers by Bayesian Classifier
المؤلفون: Luisa Matiz-Ceron, Alejandro Reyes, Juan Anzola
المصدر: Frontiers in Plant Science, Vol 12 (2022)
بيانات النشر: Frontiers Media S.A.
سنة النشر: 2022
المجموعة: Directory of Open Access Journals: DOAJ Articles
مصطلحات موضوعية: Naïve Bayesian classifier, metabarcoding, matK, trnL, taxonomic classification, chloroplast, Plant culture, SB1-1110
الوصف: DNA barcodes are standardized sequences that range between 400 and 800 bp, vary at different taxonomic levels, and make it possible to assign sequences to species that have been previously taxonomically characterized. Several DNA barcodes have been postulated for plants, nonetheless, their classification potential has not been evaluated for metabarcoding, and as a result, it would appear as none of them excels above the others in this area. One tool that has been widely used and served as a baseline when evaluating new approaches is Naïve Bayesian Classifiers (NBC). The present study aims at evaluating the classification power of several plant chloroplast genetic markers that have been proposed as barcodes (trnL, rpoB, rbcL, matK, psbA-trnH, and psbK) using an NBC. We performed the classification at different taxonomic levels, and identified problematic genera when resolution was desired. We propose matK and trnL as potential candidate markers with resolution up to genus level. Some problematic genera within certain families could lead to the misclassification no matter which marker is used (i.e., Aegilops, Gueldenstaedtia, Helianthus, Oryza, Shorea, Thysananthus, and Triticum). Finally, we suggest recommendations for the taxonomic identification of plants in samples with potential mixtures.
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 1664-462X
Relation: https://www.frontiersin.org/articles/10.3389/fpls.2021.782663/full; https://doaj.org/toc/1664-462X; https://doaj.org/article/41f8451b3fb64726b06b27f53f953c25
DOI: 10.3389/fpls.2021.782663
الاتاحة: https://doi.org/10.3389/fpls.2021.782663
https://doaj.org/article/41f8451b3fb64726b06b27f53f953c25
رقم الانضمام: edsbas.DD39500D
قاعدة البيانات: BASE
الوصف
تدمد:1664462X
DOI:10.3389/fpls.2021.782663