Academic Journal
Characterization of the molecular mechanisms of silicon uptake in coccolithophores
| العنوان: | Characterization of the molecular mechanisms of silicon uptake in coccolithophores |
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| المؤلفون: | Ratcliffe, Sarah, Meyer, Erin M, Walker, Charlotte E, Knight, Michael, McNair, Heather M, Matson, Paul G, Iglesias-Rodriguez, Debora, Brzezinski, Mark, Langer, Gerald, Sadekov, Aleksey, Greaves, Mervyn, Brownlee, Colin, Curnow, Paul, Taylor, Alison R, Wheeler, Glen L |
| المصدر: | Ratcliffe , S , Meyer , E M , Walker , C E , Knight , M , McNair , H M , Matson , P G , Iglesias-Rodriguez , D , Brzezinski , M , Langer , G , Sadekov , A , Greaves , M , Brownlee , C , Curnow , P , Taylor , A R & Wheeler , G L 2022 , ' Characterization of the molecular mechanisms of silicon uptake in coccolithophores ' , Environmental Microbiology . https://doi.org/10.1111/1462-2920.16280 |
| سنة النشر: | 2022 |
| المجموعة: | University of Bristol: Bristol Reserach |
| مصطلحات موضوعية: | /dk/atira/pure/core/keywords/biodesign_SRI, Bristol BioDesign Institute |
| الوصف: | Coccolithophores are an important group of calcifying marine phytoplankton. Although coccolithophores are not silicified, some species exhibit a requirement for Si in the calcification process. These species also possess a novel protein (SITL) that resembles the SIT family of Si transporters found in diatoms. However, the nature of Si transport in coccolithophores is not yet known, making it difficult to determine the wider role of Si in coccolithophore biology. Here, we show that coccolithophore SITLs act as Na+ -coupled Si transporters when expressed in heterologous systems and exhibit similar characteristics to diatom SITs. We find that CbSITL from Coccolithus braarudii is transcriptionally regulated by Si availability and is expressed in environmental coccolithophore populations. However, the Si requirement of C. braarudii and other coccolithophores is very low, with transport rates of exogenous Si below the level of detection in sensitive assays of Si transport. As coccoliths contain only low levels of Si, we propose that Si acts to support the calcification process, rather than forming a structural component of the coccolith itself. Si is therefore acting as a micronutrient in coccolithophores and natural populations are only likely to experience Si limitation in circumstances where dissolved silicon (DSi) is depleted to extreme levels. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1111/1462-2920.16280 |
| الاتاحة: | https://hdl.handle.net/1983/a45bacf0-a88f-4501-98cf-b24c30feb07d https://research-information.bris.ac.uk/en/publications/a45bacf0-a88f-4501-98cf-b24c30feb07d https://doi.org/10.1111/1462-2920.16280 https://research-information.bris.ac.uk/ws/files/348407273/Environmental_Microbiology_2022_Ratcliffe_Characterization_of_the_molecular_mechanisms_of_silicon_uptake_in.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DD2CDAA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/1462-2920.16280 |
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