Academic Journal
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis
| العنوان: | Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
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| المؤلفون: | Rocha, Katya C., Pinhal, Cinthia, Cavalcanti, Sonia, Vidal, Monica S. M., Toscano, Matheus, Moraes-Vasconcelos, Dewton, Duarte, Alberto J. S., Fonseca, Fernando L. A., Abreu, Luiz Carlos de, Valenti, Vitor Engrácia, Grumach, Anete S. G. |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| بيانات النشر: | Biomed Central Ltd. |
| سنة النشر: | 2012 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Lymphocytes, Antigens, Biases, Statistical, Cryptococcus, Methods, Cryptococcus neoformans |
| الوصف: | Faculdade de Medicina, Universidade de São Paulo ; Universidade Estadual Paulista ; Background: Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a specific lymphocyte transformation assay to Cryptococcus neoformans in order to identify immunodeficiency associated to neurocryptococcosis (NCC) as primary cause of the mycosis.Methods: Healthy volunteers, poultry growers, and HIV-seronegative patients with neurocryptococcosis were tested for cellular immune response. Cryptococcal meningitis was diagnosed by India ink staining of cerebrospinal fluid and cryptococcal antigen test (Immunomycol-Inc, SP, Brazil). Isolated peripheral blood mononuclear cells were stimulated with C. neoformans antigen, C. albicans antigen, and pokeweed mitogen. The amount of H-3-thymidine incorporated was assessed, and the results were expressed as stimulation index (SI) and log SI, sensitivity, specificity, and cut-off value (receiver operating characteristics curve). We applied unpaired Student t tests to compare data and considered significant differences for p<0.05.Results: The lymphotoxin alpha showed a low capacity with all the stimuli for classifying patients as responders and non-responders. Lymphotoxin alpha stimulated by heated-killed antigen from patients with neurocryptococcosis was not affected by TCD4+ cell count, and the intensity of response did not correlate with the clinical evolution of neurocryptococcosis.Conclusion: Response to lymphocyte transformation assay should be analyzed based on a normal range and using more than one stimulator. The use of a cut-off value to classify patients with neurocryptococcosis is inadequate. Statistical analysis should be based on the log transformation of SI. A more purified antigen for evaluating specific response to C. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2334 |
| Relation: | BMC Infectious Diseases; 2.620; 1,576; http://dx.doi.org/10.1186/1471-2334-12-278; Bmc Infectious Diseases. London: Biomed Central Ltd., v. 12, p. 8, 2012.; http://hdl.handle.net/11449/10767; WOS:000314094100001; WOS000314094100001.pdf |
| DOI: | 10.1186/1471-2334-12-278 |
| الاتاحة: | http://hdl.handle.net/11449/10767 https://doi.org/10.1186/1471-2334-12-278 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.DD2B87C5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712334 |
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| DOI: | 10.1186/1471-2334-12-278 |