التفاصيل البيبلوغرافية
| العنوان: |
The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation |
| المؤلفون: |
Carreras Sureda, Amado, Zhang, Xin, Laubry, Loann, Brunetti, Jessica, Konig, Stéphane, Wang, Xiaoxiao, Castelbou, Cyril, Hetz, Claudio, Liu, Yong, Frieden, Maud, Demaurex, Nicolas |
| المصدر: |
ISSN: 2211-1247 ; Cell reports, vol. 42, no. 12 (2023) 113540. |
| سنة النشر: |
2023 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/612, CP: Cell biology, CP: Immunology, CRISPR screening, CaMPARI, ER stress, IRE1, SOCE, STIM1, T cells, Calcium, Muscle, Humans, Calcium / metabolism, Calcium Channels / metabolism, Calcium Signaling / physiology, Cell Membrane / metabolism, Neoplasm Proteins / metabolism, ORAI1 Protein / metabolism, Protein Serine-Threonine Kinases / metabolism, Stromal Interaction Molecule 1 / metabolism |
| الوصف: |
Store-operated Ca 2+ entry (SOCE) mediated by stromal interacting molecule (STIM)-gated ORAI channels at endoplasmic reticulum (ER) and plasma membrane (PM) contact sites maintains adequate levels of Ca 2+ within the ER lumen during Ca 2+ signaling. Disruption of ER Ca 2+ homeostasis activates the unfolded protein response (UPR) to restore proteostasis. Here, we report that the UPR transducer inositol-requiring enzyme 1 (IRE1) interacts with STIM1, promotes ER-PM contact sites, and enhances SOCE. IRE1 deficiency reduces T cell activation and human myoblast differentiation. In turn, STIM1 deficiency reduces IRE1 signaling after store depletion. Using a CaMPARI2-based Ca 2+ genome-wide screen, we identify CAMKG2 and slc105a as SOCE enhancers during ER stress. Our findings unveil a direct crosstalk between SOCE and UPR via IRE1, acting as key regulator of ER Ca 2+ and proteostasis in T cells and muscles. Under ER stress, this IRE1-STIM1 axis boosts SOCE to preserve immune cell functions, a pathway that could be targeted for cancer immunotherapy. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/38060449; info:eu-repo/semantics/dataset/url/https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD046579; info:eu-repo/semantics/dataset/url/https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-13548?query=e-mtab-13548; info:eu-repo/semantics/dataset/url/https://data.mendeley.com/datasets/4zyx9gbhzt/1; https://archive-ouverte.unige.ch/unige:177196; unige:177196 |
| الاتاحة: |
https://archive-ouverte.unige.ch/unige:177196 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.DCB4B57B |
| قاعدة البيانات: |
BASE |