Academic Journal
Conditioned medium from amniotic membrane-derived cells prevents lung fibrosis and preserves blood gas exchanges in bleomycin-injured mice-specificity of the effects and insights into possible mechanisms
| العنوان: | Conditioned medium from amniotic membrane-derived cells prevents lung fibrosis and preserves blood gas exchanges in bleomycin-injured mice-specificity of the effects and insights into possible mechanisms |
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| المؤلفون: | A. Cargnoni, E. C. Piccinelli, L. Ressel, D. Rossi, M. Magatti, O. Parolini, I. Toschi, V. Cesari, M. Albertini, S. Mazzola |
| المساهمون: | A. Cargnoni, E.C. Piccinelli, L. Ressel, D. Rossi, M. Magatti, I. Toschi, V. Cesari, M. Albertini, S. Mazzola, O. Parolini |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2014 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | Amniotic membrane-derived cell, Amniotic mesenchymal tissue cell, Conditioned medium, Human term placenta, Lung fibrosi, Mesenchymal stromal cell, Settore VET/02 - Fisiologia Veterinaria, Settore MED/10 - Malattie dell'Apparato Respiratorio, Settore BIO/13 - Biologia Applicata |
| الوصف: | BACKGROUND AND AIMS: We recently demonstrated that injection of conditioned medium (CM) generated from cells of the mesenchymal region of human amniotic membrane (AMTCs) reduces bleomycin-induced lung fibrosis in mice, suggesting a crucial role of paracrine factor(s) secreted by AMTCs in these beneficial effects. We further investigated this hypothesis, the mechanisms involved, the effects on some lung functional parameters and whether AMTC-secreted effector(s) are specific to these cells and not produced by other cell types, extending the time of analysis up to 28 days after treatment. METHODS: Bleomycin-challenged mice were either treated with AMTC-CM or CM generated from human skin fibroblasts, human peripheral blood mononuclear cells or Jurkat cells, or were left untreated. Mouse lungs were analyzed for content of pro-inflammatory and pro-fibrotic molecules, presence of lymphocytes and macrophages and for fibrosis level (through histological semi-quantitative evaluation and quantitative measurement of collagen content). Arterial blood gas analysis was also performed. RESULTS: Up to 28 days after delivery, AMTC-CM-treated mice developed reduced lung fibrosis with respect to mice treated with other CM types. AMTC-CM-treated mice had comparatively better preservation of blood gas parameters and showed lower lung content of interleukin-6, tumor necrosis factor-α, macrophage inflammatory protein-1α, monocyte chemoattractant protein-1 and transforming growth factor-β associated with reduced lung macrophage levels. CONCLUSIONS: AMTC-CM prevents lung fibrosis in bleomycin-challenged mice, improving survival and preserving lung functional parameters such as blood gas exchanges. The specificity of AMTC-CM action was indicated by the absence of fibrosis reduction when other CM types were used. Finally, we provide some insights into the possible mechanisms underlying AMTC-CM-mediated control of fibrosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/24094500; info:eu-repo/semantics/altIdentifier/wos/WOS:000329592800003; volume:16; issue:1; firstpage:17; lastpage:32; numberofpages:16; journal:CYTOTHERAPY; http://hdl.handle.net/2434/235212; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84890983425 |
| DOI: | 10.1016/j.jcyt.2013.07.002 |
| الاتاحة: | http://hdl.handle.net/2434/235212 https://doi.org/10.1016/j.jcyt.2013.07.002 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.DC950A96 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jcyt.2013.07.002 |
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