Academic Journal
Increased 5-Lipoxygenase Immunoreactivity in the Hippocampus of Patients With Alzheimer's Disease
| العنوان: | Increased 5-Lipoxygenase Immunoreactivity in the Hippocampus of Patients With Alzheimer's Disease |
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| المؤلفون: | Ikonomovic, Milos D., Abrahamson, Eric E., Uz, Tolga, Manev, Hari, DeKosky, Steven T. |
| المصدر: | Journal of Histochemistry & Cytochemistry ; volume 56, issue 12, page 1065-1073 ; ISSN 0022-1554 1551-5044 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2008 |
| الوصف: | The proinflammatory enzyme 5-lipoxygenase (5-LOX) is upregulated in Alzheimer's disease (AD), but its localization and association with the hallmark lesions of the disease, β-amyloid (Aβ) plaques and neurofibrillary tangles (NFTs), is unknown. This study examined the distribution and cellular localization of 5-LOX in the medial temporal lobe from AD and control subjects. The spatial relationship between 5-LOX immunoreactive structures and AD lesions was also examined. We report that, in AD subjects, 5-LOX immunoreactivity is elevated relative to controls, and its localization is dependent on the antibody-targeted portion of the 5-LOX amino acid sequence. Carboxy terminus-directed antibodies detected 5-LOX in glial cells and neurons, but less frequently in neurons with dystrophic (NFT) morphology. In contrast, immunoreactivity observed using 5-LOX amino terminus-directed antibodies was virtually absent in neurons and abundant in NFTs, neuritic plaques, and glia. Double-labeling studies showed a close association of 5-LOX-immunoreactive processes and glial cells with Aβ immunoreactive plaques and vasculature and also detected 5-LOX in tau immunoreactive and amyloid containing NFTs. Different immunolabeling patterns with antibodies against carboxy vs amino terminus of 5-LOX may be caused by post-translational modifications of 5-LOX protein in Aβ plaques and NFTs. The relationship between elevated intracellular 5-LOX and hallmark AD pathological lesions provides further evidence that neuroinflammatory pathways contribute to the pathogenesis of AD. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1369/jhc.2008.951855 |
| الاتاحة: | https://doi.org/10.1369/jhc.2008.951855 https://journals.sagepub.com/doi/pdf/10.1369/jhc.2008.951855 https://journals.sagepub.com/doi/full-xml/10.1369/jhc.2008.951855 |
| Rights: | https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.DC77BBE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1369/jhc.2008.951855 |
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